The effect of different levels of drought stress in greenhouse condition on the expression of Germacron A synthase (TpGAS) and Parthenolide synthetase (TpPTS) genes involved in the production of Parthenolide in Tanacetum parthenium L.

Seyyedeh Samaneh Mosavi Khorshidi ¹ ( Ph.D. Student, Islamic Azad University, Branch of Damghan, Damghan, Iran )

Submited date : 2017-09-23 Accepted date : 2017-09-26

Keywords: Drought stress, TpGAS, Parthenolide, Tanacetum parthenium L, TpPTS,

Abstract :

Feverfew (Tanacetum parthenium) is a herbaceous plant with antimicrobial, antiseptic, anti-parasitic and anti-inflammatory effects. Parthenolide is the most important ingredient of 30 known sesquiterpene lactone in feverfew. Parthenolide is a germacranolide that its pharmacological activity is highly regarded. In the present study, young leaves of feverfew that were under different levels of stress were investigated in order to study key genes expression changes of parthenolide biosynthetic pathway includes germacrene A synthase (TpGAS) and parthenolide synthase (TpPTS) by Real time PCR. The parthenolide amount was measured through the extraction method with HPLC and parthenolide standard curve. The results showed a significant increase in gene expression of germacrene A synthase and parthenolide synthase in the leaves of plants under drought stress showed a significant increase than the control plants. The highest expression of germacrene A synthase and parthenolide synthase was occurred while the plant was under severe drought stress (irrigation once every 7 days). The changes trend in gene expression was consistent with the changes trend of parthenolide amount.