Evaluation of the effect of Azospirillum spp. inoculation, alone and in combination with phosphate solubilizing bacteria Bacillus megaterium , on alleviation of the effects of salinity stress on Mung bean (Vigna radiata L.)
Subject Areas : GeneticNosratollah Abasi 1 , Jalal Jalilian 2 , Mohhamad Javad Zare 3
1 - Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Urmia, Iran
2 - Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Urmia, Iran
3 - Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Ilam, Iran
Keywords: Yield, Salinity, biofertilizer, Mung bean, Nutrient absorption,
Abstract :
Increasing salinity of soil and water is the most important concern for agriculture in dry lands. In this research the effect of Azospirillum inoculation alone and in co-inoculation with phosphate solubilizing bacteria was investigated in alleviating the adverse effect of salinity on mung bean Vigna radiata L. cv Gohar) for 2-year under field condition. The study was a factorial experiment based on a randomized complete block design with three replicates. The main factors were application of various serovars of Azospirillum, phosphate solubilizing bacteria Bacillus megaterium, co-inoculation of both bacteria, non-inoculated treatment as control and two levels of salinity irrigation (0.2 and 12 dS m-1). Analysis of variance indicated significant effect of salinity and microbial inoculation on mung bean seed yield. Salinity resulted in reduction of seed yield by 88%. Under salinity and non-salinity conditions, the highest amount of grain yield, seed weight, and seed number was observed in plants with seeds inoculated with Azospirillum. The minimum Na and K and maximum P contents of seeds were observed in Azospirillum inoculated plants. Co-inoculation with Azospirillum and Bacillus megaterium resulted in no further beneficial effect under saline and non-saline conditions. Overall, Azospirillum appeared to be effective in mitigation of the adverse effect of NaCl and its co-inoculation with phosphate solubilizing bacteria gained no more additives benefit.
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