The effect of slope direction on genetic diversity of Juniperus polycarpos C. Koch Chahar Bagh habitat in Golestan province through biochemical markers

Subject Areas : Genetic

yasna jalili ¹ , Davood Azad far ² , zohre saeedi ³

1 - Department of Forestry,Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
2 - Department of Forestry,Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
3 - Department of Forestry,Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

Received: 2017-09-26 Accepted : 2017-12-04 Published : 2017-09-23

Keywords: Charbagh, Genetic variation, Enzyme marker, Slope direction, Jniperus polycarpos,

Abstract :

This research aimed to study the genetic diversity of trees in Chahar Bagh habitat in Golestan province using peroxidase and esterase enzymes in both slope directions. To investigate the genetic diversity of Juniperus polycarpos species, 48 trees from both the East and the West slope direction of Chahar Bagh habitat were selected. Scales samples were sampled at the same height from the ground to the tree canopy. After extraction of the samples, qualitative study of the enzyme was carried out using Polyacrylamide gel electrophoresis (PAGE). Cluster analysis of the qualitative data showed the diversity of the intra population in the East and the West in 5 and  6 Groups respectively and the inter population variation in six groups. Quality of peroxidase and esterase activity scales showed that the mean observed number of alleles, mean effective number of alleles, mean gene diversity (Ney), mean Shannon Information Index, percentage of polymorphism and mean polymorphic information content (PIC) in the slope direction of the East were more than that oof the West. Comparison of genetic diversity of inter population and intra populations genetic diversity of Juniperus  polycarpos species suggested that the inter and intra population genetic diversity are almost the same. The findings of this study highlight the need for conservation and utilization of effective methods to improve the genetic diversity of this species.

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