The effect of pumpkin seeds hydroalcoholic extract and endurance training on mitochondrial biogenesis markers and DNA damage in uterine tissue in rats toxicated by hydrogen peroxide

Subject Areas : Open Access

Shahrzad Asghari ¹ , mohammadali azarbayjani ² , maghsoud peeri ³ , hasan matin homaee ⁴

1 - No 69, karimpoor street No 10
2 - department faculty
3 - department faculty
4 - Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran

Received: 2023-08-23 Accepted : 2023-09-18 Published : 2023-05-22

Keywords: Aerobic training, Mitochondrial biogenesis, pumpkin seed, uterine,

Abstract :

AbstractIntroduction: The imbalance in the prooxidant-antioxidant ratio causes uterine tissue damage. The aim of this study was to evaluate the effect of Pumpkin seed and aerobic training on mitochondrial biogenesis markers and DNA damage in uterine tissue in rats toxicated by hydrogen peroxide. Method: To implementation of this experimental research, 42 male wistar rats randomly were divided into 7 groups. All groups received 100 mg/kg body weight of hydrogen peroxide (H2O2) for 14 days intraperitoneal. Rats in supplemented groups received hydrophilic the rats received supplemental hydroalcoholic extract of pumpkin seeds at doses of 1 and 2 mg/kg body weight per day by gavage method. Aerobic training was performed on a treadmill at a speed of 23 m/min, 30 min/day, 5 days/week for eight weeks. 24 hours after the last training session, rat uterine tissue was collected. Data were analyzed using by Independent T-test, two-way ANOVA and Bonferroni post hoc test at P<0.05. Results: Combined intervention of pumpkin seed extract with aerobic exercise significantly increased methyl guanine levels and oxidant-prooxidant balance (PAB), as well as significantly decreased adenosine triphosphate (ATP) and malondialdehyde (MDA) levels in uterine tissue (P<0.05).Conclusion: According to the results, supplementation of pumpkin seed with aerobic exercise can have a greater effect on improving mitochondrial biogenesis and reducing uterine tissue DNA damage.