Prevalence of verotoxigenic Escherichia coli (VTEC) on slaughtered cattle and calves in Tabriz abattoir

Subject Areas : Veterinary Clinical Pathology

منصور Khakpour ¹ , فرهاد Nazeri ² , جلیل Khandagi ³ , جلال Shaieg ⁴

1 - Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2 - Ggraduate of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
3 - Department of Food Industry, Sarab Branch, Islamic Azad University, Sarab, Iran
4 - Department of Pathobiology, Faculty of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran

Received: 2011-02-14 Accepted : 2011-06-08 Published : 2012-02-20

Keywords: PCR, Cow, Verotoxicogenic E.coli, Calve, Tabriz slaughterhouse,

Abstract :

E.coli is natural flour in large intestine and main common pathogen between human and animal , and its transportable by food , that its important cause of diarrhea infections in cows , particularly in calves. The aim of this survey is identify and separating E.coli and verotoxicogenic, that by referring to Tabriz slaughterhouse in chance 43 excrement samples from calves and 151 excrement samples from cows has been taken and transport to microbiology laboratory of veterinary faculty of Islamic azad TabrizUniversity. After in riching the samples and culturing in plates this results has been taken: From 194 taken samples, 113 E.coli samples were separated that were 85 positive sorbitol samples and 28 negative sorbitol samples .From 85 positive sorbitol samples were 19 samples of calves and 66 samples of cows and from 28 negative sorbitol samples were 13 samples of calves and 15 samples of cows. Also serological test accomplished for determining E.coli non O157 serotypes on the positive sorbitol samples that 9 samples of cows and 42 samples of cows demonstrate positive reaction, then on 28 negative sorbitol samples and 85 positive sorbitol samples, the PCR test accomplished by using secans of stx1, stx2 gens, that 15 samples of 28 negative sorbitol samples and 19 samples of 42 positive sorbitol samples were E.coli and verotoxicogenic. The result demonstrated that high amount of E.coli and verotoxicogenic exist in excrement of cows and calves slaughtered in Tabriz slaughterhouse.  

References:

1. جاوتز، 2001. میکروب شناسی پزشکی، ترجمه ارجمند، م. و ستوده نیا، ع.، انتشارات ارجمند، صفحات: 346-326.


2. طباطبایی، ع. و فیروزی، ر. 1380. بیماری­های باکتریایی دام، انتشارات دانشگاه تهران، صفحات: 231- 206.


3. کوئین،  مارکی،  کارتر، دونلی، لئونارد، 1386. میکروب شناسی دامپزشکی و بیماری­های میکروبی، ترجمه زهرایی صالحی، ت. و  شایق، ج.، انتشارات دانشگاه تهران، صفحات: 199-157.


4. مک فادین، جی. 1383. آزمایش­های بیوشیمیایی برای شناسایی باکتری­های پزشکی، ترجمه رحمتی، ا.، انتشارات دانشگاه علوم پزشکی تبریز. صفحات: 804-873 .
   
   
   1. Blanco, J., Gonzalez, E.A., Garcia, S., Blanco, M., Regueiro, B., Bernardez, I. 1988. Production of toxins by Escherichia coli strains isolated from calves with diarrehoea in Galicia (North-western Spain), Veterinary Microbiology, Vol 18, pp: 297-311.
   
   
   2. Fukushima, H. and Seki, R. 2004. High numbers of shiga toxin-producing Escherichia coli found in bovine feces collected at slaughter in Japan, FEMS Microbiology Letters, Vol 238, pp: 189-197.
   
   
   3. Gannon, V.P.J., D’souza,S., Graham, T., King, R.K.,  Rahn, K. and Read, S. 1996. Use of the Flagellar H7 Gene as a Target in Multiplex PCR Assays and Improved Specificity in Identification of Enterohemorrhagic Escherichia coli Strains, Animal Diseases Research Institute, Agriculture and Agri-Food Canada, Canada T1J 3Z4, Canada N1G 3W4.
   
   
   4. Laven, R.A., Ashmore, A. and Stewart, C.S. 2003. Escherichia coli in the Rumen and Colon of Slauthter Cattle , with Particular reference to E.coli O157, The Veterinary journal, Vol 165 , pp: 78-83.
   
   
   5. Muffling, T.V., Smaijlovic, M., Nowak, B., Sammet, K., Bulte, M. and Klein, G. 2007. Preliminary study of certain serotypes, genetic and antimicrobial resistance profiles of verotoxicogenic Escherichia coli(VTEC) isolated in Bosnia and Germany from cattle or pigs and their products, International Journal of Food Microbiology, Vol 117, pp: 185-191.
   
   
   6. Sergeant, J.M., Sanderson, M.W., Smith, R.A., Dee Griffin, D. 2004. Associations between management , climate , and Escherichia coli O157 in the feces of feedlot cattle in the Midwestern USA, Preventive Veterinary Medicine , Vol 66, pp: 175-206 .