Prevalence of verotoxigenic Escherichia coli (VTEC) on slaughtered cattle and calves in Tabriz abattoir
Subject Areas : Veterinary Clinical Pathology
منصور Khakpour
1
(
Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
)
فرهاد Nazeri
2
(
Ggraduate of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
)
جلیل Khandagi
3
(
Department of Food Industry, Sarab Branch, Islamic Azad University, Sarab, Iran
)
جلال Shaieg
4
(
Department of Pathobiology, Faculty of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran
)
Keywords: PCR, Cow, Verotoxicogenic E.coli, Calve, Tabriz slaughterhouse,
Abstract :
E.coli is natural flour in large intestine and main common pathogen between human and animal , and its transportable by food , that its important cause of diarrhea infections in cows , particularly in calves. The aim of this survey is identify and separating E.coli and verotoxicogenic, that by referring to Tabriz slaughterhouse in chance 43 excrement samples from calves and 151 excrement samples from cows has been taken and transport to microbiology laboratory of veterinary faculty of Islamic azad TabrizUniversity. After in riching the samples and culturing in plates this results has been taken: From 194 taken samples, 113 E.coli samples were separated that were 85 positive sorbitol samples and 28 negative sorbitol samples .From 85 positive sorbitol samples were 19 samples of calves and 66 samples of cows and from 28 negative sorbitol samples were 13 samples of calves and 15 samples of cows. Also serological test accomplished for determining E.coli non O157 serotypes on the positive sorbitol samples that 9 samples of cows and 42 samples of cows demonstrate positive reaction, then on 28 negative sorbitol samples and 85 positive sorbitol samples, the PCR test accomplished by using secans of stx1, stx2 gens, that 15 samples of 28 negative sorbitol samples and 19 samples of 42 positive sorbitol samples were E.coli and verotoxicogenic. The result demonstrated that high amount of E.coli and verotoxicogenic exist in excrement of cows and calves slaughtered in Tabriz slaughterhouse.
