Evaluation of serumic Uric acid,Total Protein, Albumin, Calcium and Phosphorus levels in laying hens afflicted by the gout syndrome

Subject Areas : Veterinary Clinical Pathology

بهرام Amouoghli Tabrizi ¹ ( Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran )
حسین Nikpiran ² ( Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran )
SH Halabi ³ ( Graduate of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran )

Received: 2010-09-27 Accepted : 2011-02-15 Published : 2011-02-20

Keywords: total protein, Calcium, Layer, phosphorus, albumin, Gout, Uric acid,

Abstract :

Gout syndrome is accounted for one of the prevalent causes of mortality in poultry farms. Gout is recognized by deposition of urates in kidneys, on serous surfaces of the heart, liver, mesenteries, air sacs and peritoneum. In some cases, these deposits may be observed on surfaces of muscles, synovial sheaths of tendons and joints, too. These deposits are like chalky coating and appear grossly. Gout could be seen in two forms, articular gout and visceral gout, and it is a disease with numerous causes.  The causing factors of the gout are classified in three groups which consist of nutrient and management factors, infectious factors, and toxic factors. For evaluation of biochemical parameters in gout disease, we surveyed and diagnosed in three farms of egg laying poultry breeding (HY-Line) affected to gout syndrome during spring and summer of 1386. In this study, from the above- mentioned poultry farms a number of 20 affected, and 20 healthy chickens (totally 120) were chosen and Blood samples of all animals were collected from wing veins and their sera were harvested by centrifuging and the levels of Uric acid, Total Protein, Albumin, Calcium and Phosphors parameters were measured by diagnostic laboratory kits. After taking blood, they were autopsied, and according to autopsy signs and syndromes, the diagnosis of gout was confirmed. Results of this study showed  significant increase on mean serumic Uric acid, Total Protein, Albumin, Calcium and Phosphorus in chickens affected by gout in comparison with the  healthy groups(p<0.05). In conclusion, with the measurement of these parameters in the laboratory, one could diagnose the gout syndrome even before the clinical signs become apparent. 

References:

1. ریدل، س.،  خداکرم تفتی، ع. و مرجانمهر، م 1376. هیستوپاتولوژی پرندگان، دانشگاه شیراز، صفحه: 206.


2. مجابی، ع. 1384. بیوشیمی بالینی دامپزشکی، انتشارات نوربخش.


3. مراد رحیمی، ع. 1376. صدمات نقرس احشایی به مرغ­های تخم­گذار و گوشتی، چکاوک، شماره 36.


4. منصوری‌نیا، ی. و فیضی، ع. 1383. بررسی سندرم نقرس در تعدادی از مرغداری­های گوشتی و تخم­گذار تبریز در سال 83-82، پایان‌نامه دکتری حرفه‌ای دامپزشکی دانشگاه آزاد اسلامی تبریز،‌ دانشکده دامپزشکی. ‌
   
   
   1. Austic, R. and Cole, R. 2004. Imparived read clearance of uric acid in chickens having hyperuricamia and articular goat. Am. J. Physiol. 223:525-530.
   
   
   2. Beckman, B. 2000. Avian urolithiasis (Renal or visceralgout), Technical Bulliten Hy-line international. 9:151-175.
   
   
   3. Bertelli, M.A., Randi, D.M., Micheli, S.O, Carelli, M.A. and Pandolfo, M.C. 2004. Molecular basis of hypoxauthine-guanine phosphoribosyltrans Ferase deficiency in Italian Lesch-Nyhan patients. Kluwer Academic in the Netherlands. 27:767-773.  
   
   
   4. Bodo, E., Knudsen, D., Darren, T., Beiko, R. and Jobn, D. 1998. Uric Acid Urolithiasis. Poult. Sci. 16:299-315.
   
   
   5. Cole, R. and Austic, R. 1980. Hereditary uricemia and articular gout in chickens. Poult Sci. 59: 951-975.
   
   
   6. Jordan. F., Pattison M., Alexander. D. and Faragher, T. 2002. Poultry Diseases. Fifth Edition. p: 356-394.     
   
   
   7. Julian, R. 1987. The effect of increased sodium in drinking water on right ventricular hypertrophy, right ventricular failure in broiler chickens. Avian pathol. 16:61-77.
   
   
   8. Melissa, A. Williams, C. and Twine, J. 2001. Nephrolithiasis. Springer-verlage, Berlin. 15:256-259.
   
   
   9. Peinado, V., Polo, F., Viscor, G. and Palmeque, J. 1992. Haematology and blood chemistry values for several flamingo species. Avi Pathol. 21:55-64.
   
   
   10. Romanoff, A. 1967. Biochemistry of the avian embryo. A quantitative and lysis of prenatal development. John wiley and Sons. New York, Sydney. p: 300-340.
   
   
   11. Shivaprasad, H. 1998. An overwies of anatomy, physiology and pathology of urinary system in birds, AAV proceedings. p: 201-205.
   
   
   12. Sholsberg, A., Elkin, N., Malkinson, M., Orgad, U., Bogin, E., Weisman, Y., et al. 1997. Sever hepatopathy in geese and broilers associated with ochratoxin in their feed. Kluwer Academic Publishers. 138:71-76.
   
   
   13. Tietz, N. 1986. Textbook of Clinical Chemistry. 1st ed. W.B. Saunders Co. Philadelphia. p: 327-338, 356-377.