Serum Levels of Zinc, Copper and Their Carrier Proteins in Cattle with theileriosis

Subject Areas : Veterinary Clinical Pathology

مجید Fartashvand ¹ , بهرام Amouoghli Tabrizi ² , علی Hasanpour ³ , سید رضا S.J ⁴ , SH Pourhassan ⁵ , یعقوب Haji-Sadeghi ⁶

1 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
3 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
4 - اداره کل دامپزشکی استان آذربایجان شرقی، شهرستان کلیبر
5 - Large Animal Special Clinic, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
6 - Student of Veterinary Medicine, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran

Received: 2010-05-02 Accepted : 2011-02-15 Published : 2011-02-20

Keywords: Theileriosis, Zinc (Zn), Copper (Cu), Ceruloplasmin, Transferrin,

Abstract :

In this study, 90 cattle with theileriosis and 90 healthy cattle were studied based on clinical and laboratory examination including parasitological and biochemical tests. Special biochemical kits were used for determination of zinc, copper, albumin, calcium, magnesium and ferrous levels in sera. Serum levels of transferin and ceruloplasmin were measured with ELISA and Sunderman & Nomoto method, respectively. The serum level of zinc was significantly decreased in cattle suffering from theileriosis (p<0.01). Serum concentration of copper showed decrease in cattle with theileriosis but it was not significant. The Biochemical findings revealed that cattle infected with theileriosis have significantly lower serum levels of transferin, magnesium, albumin, iron (p<0.01) and calcium (p<0.05). Serum value of ceruloplasmin in sick animals was significantly more than healthy cattle (p<0.05). There was significant relationship between serum level of zinc and ceruloplasmin with PCV and also between serum iron level and parasitemia (p<0.05). Results obtained from measurement of biochemical parameters in serum suggested that reduction of serum zinc and copper may be one of the possible reasons of immunosuppression and incidence of secondary infections in cattle with theileriosis. Therefore use of these trace elements, especially zinc in treatment protocol of infected cattle may be beneficial.

References:

1. اصلانی، م.ر.، رضاخانی، ع. و احمدی حریری، ع.ر. 1377. مطالعه تغییرات پارامترهای هماتولوژیک، الکترولیتها و بیلی­روبین سرم بعد از درمان تیلریوز گاوی به وسیله بوپاروکون. پژوهش و سازندگی، شماره 39، صفحات 77-72.


2. خواجه، غ.ح.، حاجیکلایی، م.ر.، راضی جلالی، م.، راسخ، ع.ر. و علوی، ن. 1384. مطالعه برخی پارامترهای الکترولیتی و غیر الکترولیتی سرم خون گاوهای مبتلا به تیلریا آنولاتا. پژوهش و سازندگی (امور دام و آبزیان)، شماره 66، صفحات 52-46.

1. Chaturvedi, U.C., Shrivastava, R. and Upreti, R.K. 2004. Viral infections and trace elements: a complex interaction. Curr. Sci. (87):1536-1554.


2. Chaudhuri, S., Varshney, J.P. and Patra, R.C. 2008. Erythrocytic antioxidant defense, lipid peroxides level and blood iron, zinc and copper concentrations in dogs naturally infected with Babesia gibsoni. Research in Veterinary Science. 85:120-124.


3. Chesters, J.K. 1983. Zinc metabolism in animals: pathology, immunology and genetics. Journal of inherited Metabolic Disease. 6 (1):34-38.


4. Chhabra, A. and Arora, S.P. 1993. Effect of vitamin A and Zn supplement on alcohol dehydrogenase and superoxide dismutase activities of goat tissues. Indian J. Anim. Sci. 63:334-338.


5. Col, R. and Uslu, U. 2007. Changes in selected serum components in cattle naturally infected with Theileria annulata. Bull. Vet. Inst. Pulawy. 51:15-18.


6. Dede, S., Değer, Y., Değer, S. and Tanrıtanır, P. 2008. Plasma Levels of Zinc, Copper, Copper/Zinc Ratio, and Activity of Carbonic Anhydrase in Equine Piroplasmosis. Biol. Trace Elem. Res. (125):41-45.


7. Deger, S., Biçek, K. and Deger, Y. 2005. The changes in some of biochemical parameters (iron, copper, vitamin C and vitamin E) in infected cattle with theileriosis. YYU Vet. Fak. Derg. 16(1):49-50.


8. Ece, A., Uyamik, B.S., Iscan, A., Ertan, P. and Yigitoglu, M.R. 1997. Increased serum copper and decreased serum zinc levels in children with iron deficiency anemia. Biol. Trace Elem. Res. 59(1-3):31-39.


9. Failla, M.L. 2003. Trace elements and host defense: recent advances and continuing challenges. J. Nutr. (133):1443-1447.


10. Feldman, B.V., Zinkl, J.G. and Jain, N.C. 2000. Schalm's Veterinary Hematology. Williams &Wilkins, Philadelphia, 5^th edition, p: 1152.


11. Gürgöze, M.K., Olçücü, A., Aygün, A.D., Taskin, E. and Kiliç, M. 2006. Serum and hair levels of zinc, selenium, iron, and copper in children with iron-deficiency anemia. Biol. Trace Elem. Res. 111(1-3):23-29.


12. Healy, J. and Tipton, K. 2007. Ceruloplasmin and what it might do. J. Neurol. Transm. 114(6):777-781.


13. Hussein, A.H., Mohammed, N.A.-E.-S. and Mohammed, H.K. 1997. Theileriosis and babesiosis in cattle: haemogram and some biochemical parameters. IX^th International Congress on Animal Hygiene, Helsinki, Finland.


14. Jubb, K.V.F., Kenedy, P.C. and Palmer, N. 1991. Pathology of domestic animals. vol 3, Academic press, California, 4^th edition, p: 251-255.


15. Kocyigit, A., Erel, O., Gurel, M.S., Avci, S. and Aktepe, N. 1998. Alterations of serum selenium, zinc, copper and iron concentrations and, some related antioxidant enzyme activities in patients with cutaneous leishmaniasis. Biol. Trace Elem. Res. 65(3):271-281.


16. Kumar, R. and Malik, J.K. 1999. Influence of experimentally induced theileriosis (Theileria annulata) on the pharmacokinetics of a long-acting formulation of oxytetracycline (OTC-LA) in calves. Veterinary pharmacology and therapeutics, (22):320-326.


17. Lukaski, H.C. 2004. Vitamin and mineral status: effects on physical performance. Nutrition. 20(7-8):615-619.


18. Nazifi, S., Razavi, S.M., Reiszadeh, M., Esmailnezhad, Z., Ansari-Lari, M. and Hasanshahi, F. 2009. Evaluation of the resistance of indigenous Iranian cattle to Theileria annulata compared with Holstein cattle by measurement of acute phase proteins. Comp. Clin. Pathol. 19(2):155-161.


19. Omer, O.H., El-Malik, K.H., Magzoub, M., Mahmoud, O.M., Haroun, E.M., Hawas, A., and Omar, H.M. 2003. Biochemical Profiles in Friesian Cattle Naturally Infected with Theileria annulata in Saudi Arabia. Vet. Res. Comm. 27:15-25.


20. Radostitis, O.M., Gay, C.C., Hinchcliff, K.W. and Constable, P.D. 2007. Veterinary Medicine, a text book of the disease of cattle, sheep, pigs and horses. Elsevier publishing, 10^th edition. p: 1526-1532.


21. Rink, L. and Gabriel, P. 2000. Zinc and the immune system. Proceedings of the Nutrition Society. (59):541-552.


22. Sandhu, G.S., Grewal, A.S., Singh, A., Kondal, J.K., Singh, J. and Brar, R.S. 1998. Haematological and biochemical studies on experimental Theileria annulata infection in crossbred calves. Vet. Res. Comm. 22:347-354.


23. Sangwan, N. and Sangwan, A.K. 2007. Changes in blood and tissue copper levels due to Theileria annulata infection in cross-bred calves. Indian Journal of Animal Res. 41(4):157-161.


24. Sangwan, N. and Sangwan, A.K. 2007. Zinc distribution or loss in relation to progression of Theileria annulata infection in cross-bred calves. Journal of Veterinary Parasitology. 21(1):56-60.


25. Singh, A., Singh, J., Grewal, A.S. and Brar, R.S. 2001. Studies on Some Blood Parameters of Crossbred Calves with Experimental Theileria annulata Infections. Vet. Res. Comm. 25:289-300.


26. Sunderman, F.W. and Nomoto, S. 1970. Measurement of human serum ceruloplasmin by its p-Phenylenediamine oxidase activity, Clin. Chem. 16:903-910.


27. Underwood, E.J. and Suttle, N.F. 1999. The Mineral Nutrition of Livestock. CABI publishing, Wallingford, 3^rd Edition. p: 283-342, 477-513.


28. Van Weyenbergh, J., Santana, G., D’Oliveira, A.Jr., Santos, A.F.Jr., Costa, C.H., Carvalho, and et al. 2004. Zinc/copper imbalance reflects immune dysfunction in human leishmaniasis: an ex vivo and in vitro study. BMC. Infect. Dis. 4:50.


29. Watanabe, K., Ozawa, M., Ochiai, H., Kamohara, H., Iijima, N., Negita, H., and et al. 1998. Changes in Iron and Ferritin in Anemic Calves Infected with Theileria sergenti. J. Vet. Med. Sci. 60(8):943-947.


30. Wellinghausen, N., Jöchle, W., Reuter, S., Flegel, W.A., Grünert, A. and Kern, P. 1999. Zinc status in patients with alveolar echinococcosis is related to disease progression. Parasite Immunol. 21(5):237-241.


31. Yadav, C.L. and Sharma, N.N. 1986. Changes in blood chemical components during experimentally induced theileria annulata infections in cattle. Veterinary Parasitology. 21:91-98.


32. Yurtseven, S. and Uysal, H. 2009. Decreased serum sialic acid, albumin-globulin ratio and total protein levels in cattle heavily infected with Theileria annulata. Ankara Üniv. Vet. Fak. Derg. 56:141-144.