Cloning and sequencing of growth hormone gene of Iranian Lori Bakhtiari sheep

Subject Areas : Veterinary Clinical Pathology

مهدی Dayani-Nia ¹ , مرتضی Karimipoor ² , وهاب Babapoor ³ , لهراسب Shahgholian ⁴ , عباس Doosti ⁵

1 - Biotechnology Research Center, Islamic Azad University-Shahrekord Branch, Shahrekord, Iran
2 - Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
3 - Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
4 - Biotechnology Research Center, Islamic Azad University-Shahrekord Branch, Shahrekord, Iran
5 - Biotechnology Research Center, Islamic Azad University-Shahrekord Branch, Shahrekord, Iran

Received: 2009-09-26 Accepted : 2010-10-02 Published : 2010-05-22

Keywords: Sheep, cloning, Growth hormone, Nejhade Loriye Bakhtiyari,

Abstract :

Growth hormone (GH) is a peptide hormone that stimulates growth and cell reproduction in humans and animals. It is a 191-amino acid, single chain polypeptide hormone which is synthesized, stored, and secreted by the somatotroph cells within the lateral wings of the anterior pituitary gland. The goal of this research was to clone and sequence sheep growth hormone of Lori Bakhtiary breed in Iran. For this purpose, RNA was extracted from the pituitary gland of freshly slaughtered sheep and cDNA of growth hormone produced. The T/A cloning technique was used to clone the cDNA of growth hormone and then the synthesized construct was transferred into E. coli as the host. Once the correct recombinants were further confirmed by colony PCR or restriction enzyme digestion, sequencing was done. The sequencing results showed that, the length of sheep growth hormone cDNA was 690 bp fragments. Comparison of sequence of growth hormone inside the synthesized construct with those recorded in Genebank (NCBI, Blast) indicated high degrees of similarity between Iranian native sheep and other sheep breeds of the world.

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