Molecular study and nucleotide sequencing of Chlamydia abortus isolated from aborted sheep fetuses ewes of Alborz province

Subject Areas : Veterinary Clinical Pathology

amirreza  ebadi ¹ (دانش آموخته، گروه میکروبیولوژی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.)
mahmoud  jamshidian ² (استاد گروه میکروبیولوژی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.)
farhad  mousakhani ³ (استادیار گروه میکروبیولوژی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران.)

Received: 2015-02-22 Accepted : 2015-04-06 Published : 2015-02-20

Keywords: sequencing, Chlamydia abortus, Alborz,

Abstract :

   Chlamydia is an obligate intracellular and gram negative coccobacilli and one of the most important causes of abortion in ruminants especially in ewes. This investigation was performed with the purpose of molecular study and sequencing of Chlamydia abortus isolated from aborted sheep fetuses of Alborz Province. In this study, DNA extraction was performed on 100 samples from aborted fetuses of 32 sheep flocks from different areas of Alborz province. Then using specific primers of gene IGS-Sr- RNA, polymerase chain reaction was conducted and 10 samples were selected randomly from the positive cases were sent to Macrogene company in Korea for sequencing. In this study, 37 samples from a total of 100 aborted fetuses were positive for Chlamydia abortus. After sequencing, more than 99 percent of the positive samples were similar with sequences in gene bank. The sequencing results indicated that the samples were very similar to isolates LN554882/1, AF051935/1 and CR848038/1 of the gene bank and were in the same cluster. Also, this investigation indicated that Chlamydia abortus is one of the main reasons of ewe abortion in Alborz province.

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