Evaluation of serum concentration of sialic acid in foals affected by Rhodococcosis

Subject Areas : Veterinary Clinical Pathology

ali hassanpour ¹ ( دانشیار گروه علوم درمانگاهی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران. )
alireza monadi ² ( دانشیار گروه پاتوبیولوژی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران. )

Received: 2015-04-08 Accepted : 2015-12-21 Published : 2015-11-22

Keywords: Rhodococcosis, Foal, Sialic acid,

Abstract :

  This study was conducted for evaluation of sialic acid concentration in serum of 19 foals with Rhodococcosis and compared with 18 normal foals in horse stables in Tabriz area in Iran. Sick foals were detected using clinical and laboratory (culture of nasal discharge) findings. Blood samples were collected from jugular vein in all foals and serum was separated. Sialic acid (serum total sialic acid, lipid-bound sialic acid and protein-bound sialic acid) concentrations in the serum were measured in all samples. Increasing of total sialic acid, lipid-bound sialic acid and protein-bound sialic acid in the sick group was significant (p=0.000, p=0.012 and p=0.001, respectively). In the normal group, the correlation between serumic parameters was not significant. In the sick group, the correlation between total sialic acid and protein-bound sialic acid was not significant (r=0.095) but there was a significant correlation between total sialic acid and lipid-bound sialic acid (r=0.842). The correlation between lipid-bound and protein-bound sialic acid was also insignificant (r=0.196). The results obtained revealed that Rhodococcosis increases the serumic concentration of sialic acid and there is a correlation between increases in serumic concentration of various parameters.

References:

• Abramjan, E.T. and Kamaljan, V.G. (1968). Biochemical aspects in investigation of mastitis in cows. V. International meeting on diseases of cattle. September 13-17, Opatija, Yugoslavia.


• Carter, A. and Martin, N.H. (1962). Serum sialic levels in health and disease. Journal of Clinical Pathology, 15: 69-72.


• Chaffin, M.K., Cohen, N.D. and Martens, R.J. (2003). Evaluation of equine breeding farm characteristics as risk factors for development of Rhodococcus equi pneumonia in foals. Journal of American Veterinary Medicine Association, 222: 467-475.


• Chaffin, M.K., Cohen, N.D., Martens, R.J., Edwards, R.F. and Nevill, M. (2004). Foal-related risk factors for development of Rhodococcus equi on farms with endemic infections Journal of American Veterinary Medicine Association, 223: 1791-1799.


• Chaffin, M.K., Cohen, N.D., Martens, R.J., Edward, R.F. and Nevill, M. (2004). Hematologic and immunophenotypic factors associated with development of Rhodococcus equi pneumonia of foals at equine breeding farms with endemic infection. Veterinary Immunology and Immunopathology, 100: 33-48.


• Citil, M., Gunesh, V., Karapehlivan, M., Atalan, G. and Marasll, S. (2004). Evaluation of serum sialic acid as an inflammation marker in cattle with traumatic reticulo peritonitis. Revue of Médicine. Véterinary, 155(7): 389-392.


• Cohen, N.D. (2006). Treating foals with Rhodococcus equi infection: what do you recommend? Compendium on Continuing Education for the Practising Veterinarian, 1(1): 14-18.


• Deger, Y., Mert, H., Dede, S., Yur, F. and Mert, N. (2007).  Serum Total and Lipid-Bound Sialic Acid Concentrations in Sheep with Natural Babesiosis. ACTA Veterinaria Brno, 76: 379-382.


• Ertekin, A., Keles, I., Ekin, S., Karaca, M. and Akkan, H.A. (2000). An investigation on sialic acid and lipidbound sialic acid in animals with blood parasites, Van Vetrinary Journal, 11: 34-35


• Eslovo, K.A.N., Saror, D.I., Ilemobade, A.A. and Hallaway, M.H. (1982). Variation in erythrocyte Surface and free serum sialic acid concentrations during experimental Trypanosoma vivax infection in cattle. Research of Veterinary Science, 32(1): 1-5.


• Haq, M., Haq, S., Tutt, P. and Crook, M. (1993). Serum total sialic acid and lipid-associated sialic acid in normal induvals patients with myocardial infarction and their relationship to acute phase proteins. Annals of Clinical Biochemistry, 30: 383-386.  


• Harma, N.C. and Sur, B.K. (1967). Serum fucose and sialic acid levels in Indian children and adults. Under normal and pathological conditions. Indian Journal of Medical Research, 8: 380-384.


• Hassanpour, A. and Pakravani, R. (2011). Evaluation of serum sialic acid in cows with theilleriosis. Research Journal of Biological Science, 6(5): 235-237.


• Hassanpour, A., Bakhtiari, A. and Safarmashaei, S. (2012). Serumic sialic acid in calves with diarrhea syndrome. International Journal of Biological Technology, 3(1): 28-31.


• Katopodis, N., Hirshaut, Y., Geller, N.L. and Stock, C.C. (1982). Lipid-associated sialic acid test for the detection of human cancer. Cancer Research, 42: 5270-5275.


• Keles, I., Ertekin, A., Karaca, M., Ekin, S. and Akkan, H.A. (2000). Sigirlarin, leptospirozisinede serum sialik asit ve lipid-bagli sialikasit duzeyleri uzerine arastirma. Van Vetrinary Journal, 11(1): 121-122.


• Letek, M., Ocampo-Sosa, A.A., Sanders, M., Fogarty, U., Buckley, T., Leadon, D.P., et al. (2008). Evolution of the Rhodococcus equi vap pathogenicity island seen through comparison of host-associated vapA and vapB virulence plasmids. Journal of Bacteriology, 190(17): 5797-5805.


• Muscatello, G., Leadon, D.P., Klay, M., Ocampo-Sosa, A., Lewis, D.A. Fogarty, U., et al. (2007). Rhodococcus equi infection in foals. The science of 'rattles'. Equine Veterinary Journal, 39: 470-478.


• Nazifi, S.M., Ansari-lari, J., Asadi, F. and Rezaei, M. (2009). The use of receiver operating characteristic (ROC) analysis to assess the diagnostic value of serum amyloid A, haptoglobin and fibrinogen in traumatic reticuloperitonotis in cattle. Veterinary Journal, 182: 315-319.


• Radostits, O.M., Gay, C.C., Blood, D.C. and Hinchcliff, K.W. (2000). Veterinary medicine: a textbook of the diseases of cattle, sheep, pigs, goats and horses. 9th ed., Philadelphia: W.B. Saunders, pp: 800-804.


• Razi, N. and Varki, A. (1998). Masking and unmasking of the sialic acid-binding lectin activity of CD22 (Siglec-2) on B lymphocytes. Proceedings of the National Academy of Sciences of the United States of America, 95: 7469-7474.


• Schauer, R. (1982). Chemistry, metabolism and biological functions of sialic acid. Advance Carbohydrate Chemical Biochemestry, 40: 131-234.


• Stephen, M., Warwick, M., Bayly, D.C. and Sellon, S. (2004). Equine Internal Medicine. 2nd ed., Philadelphia: W.B. Saunders, pp: 429-432.


• Stefenelli, N., Klotz, H., Engel, A. and Bauer, P. (1988). Serum sialic acid in malignant tumours. Bacterial infections and chronic liver diseases. Journal of Cancer Research Clinical Oncology, 109: 55-59.


• Sydow, G., Wittamnn, W., Bender, E. and Starick, E. (1988). The sialic acid content of the serum of cattle infected with bovine leukosis virus. Archive of Experimental Veterinary Medicine, 42: 194-197.