The effect of –hydroxybutyrate and estradiol on the process of phagocytosis of ovine neutrophils in vitro

Subject Areas : Veterinary Clinical Pathology

Ali Rezapour ¹ , جعفر Majidi ² , مرتضی Tahmouzy ³

1 - Department of Animal Science, Faculty of Agriculture, Islamic Azad University, Tabriz Branch, Tabriz, Iran.
2 - Research Center of Infectious Disease, Tabriz University of Medical Science, Tabriz, Iran.
3 - Department of Animal Science, Faculty of Agriculture, Islamic Azad University, Tabriz Branch, Tabriz, Iran.

Received: 2008-10-30 Accepted : 2009-01-19 Published : 2008-11-21

Keywords: estradiol, phagocytosis, Nitroblue tetrazolium reduction test, beta-hydroxybutyrate,

Abstract :

     The object of this study was to evaluate the independent effect different concentration of beta-hydroxybutyrate (2.4 & 4.8 mmol/ml) and estradiol (20&400 pg/ml) on the functions phagocytosis and Nitroblue tetrazolium reduction test or respiratory burst of isolated ovine neutrophils in vitro. Isolated neutrophils of sheep were incubated for one hour in room temperature with the previously treatments. The study consisted of 7 treatments in 5 replicates and both phagocytosis and NBT tests of tests were performed in every treatment. The Results of this study indicated that 2.4 & 4.8 mmol/ml concentrations of beta-hydroxybutyrate and estrodiol concentrations of 20 & 400 pg/ml significantly (p<0.01) reduced the percentage of phagocytosis and NBT reduction test  of sheep neutrophils and this could be the reason for the suppression of the immune system in peripartureint  sheep.

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