Studying preventive effects of Berberisintegerrimaon root on carbon tetrachloride induced hepatotoxicity in broilers

Subject Areas : Veterinary Clinical Pathology

mohammadreza mohammadimalayeri ¹ , abolfazl dadkhahtehrani ² , zahra oraghiardebili ³ , hamid nazeri ⁴

1 - دانشگاه آزاد اسلامی، واحد گرمسار، دانشکده دامپزشکی، استادیار گروه پاتوبیولوژی، گرمسار، ایران.
2 - دانشگاه آزاد اسلامی، واحد قم، دانشکده پزشکی، استادیار گروه علوم پایه، قم، ایران.
3 - دانشگاه آزاد اسلامی، واحد گرمسار، دانشکده کشاورزی، استادیار گروه زیست شناسی، گرمسار، ایران.
4 - دانشگاه آزاد اسلامی، واحد گرمسار، دانشکده کشاورزی، دانش‌آموخته رشته کارشناسی ارشد مدیریت پرورش طیور، گرمسار، ایران.

Received: 2013-11-25 Accepted : 2014-03-03 Published : 2013-11-22

Keywords: broilers, Hepatotoxicity, Carbon tetrachloride, Berberisintegerrima,

Abstract :

Liver diseases and their economic losses have gained more importancealongside the development of integrated poultry industry. Studies have proved hepatotoxicity induced by carbon tetrachloride as one of the best experimental models of hepatotocicity. Barberries have been used widely in traditional medicine.The purpose of the present study was to evaluaterthe preventive effects of Berberisintegerrima root on carbon tetrachloride induced liver lesions in broilers.For this purpose, 80 day old Ross strain broilers were divided randomly to 8 study groupsconsisting of negative control, positive control which received IP 4ml/kg b.w. carbon tetrachloride twice in 25th and 28thdays , treatment controls consisting of 10,20 and 30 grams of  Berberisintegerrima root per kilogram of diet and treatment groups consisting of 10,20 and 30 gr. Of Berberis root / Kg diet + IPcarbontetrachloride 4ml/Kg b.w. twice in 25th and 28th days.At 29th day, blood samples were collected from animals, then they were sacrificed and their liver samples were fixed in 10% formalin solution. The blood samples were sent to laboratory to measure ALT,AST and ALP activities.Biochemical results didn't show any significant changes of ALT,AST and ALP activities between all study groups (P>0.05). Microscopic results showed significant decrease in pathologic lesions of 20 gr Berberis root /Kg diet treatment group in comparison with the positive control group(P<0.05).The results of this study indicated that to induce more severe hepatocellular lesions in broilers by carbon tetrachloride doses more than 4ml/Kg b.w are needed.Adding 20 gr Berberisintegerrima root /Kg diet could have preventive effects against acute hapatotoxicity induced by carbon tetrachloride.

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