Identification and determination of effective and immunogenic antigens of Ascaridia galli in poultry reared in Tabriz area

Subject Areas : Veterinary Clinical Pathology

Yaghob Gharehdaghi ¹ , Naser Hoghooghi Rad ² , Parviz Shayan ³ , Ali Eslami ⁴

1 - Graduated from Veterinary Parasitology, Faculty of Specialized Veterinary Sciences, Islamic Azad University, Science and Research Campus, Tehran, Iran.
2 - Department of Parasitology, Faculty of Specialized Veterinary Sciences, Islamic Azad University, Science and Research Campus, Tehran, Iran.
3 - Department of Parasitology, Faculty of Specialized Veterinary Science, Tehran Uneversity, Tehran, Iran
4 - Department of Parasitology, Faculty of Specialized Veterinary Science, Tehran Uneversity, Tehran, Iran.

Received: 2008-01-04 Accepted : 2008-01-04 Published : 2007-05-22

Keywords: Ascaridia galli, immunization, white leghorn hen,

Abstract :

In a trial, in Tabriz area, the ability of an experimental vaccine from the larval homogenate of Ascaridia galli was evaluated during the year 1384. A group of 5 white leghorn hens were immunized twice with 100 micrograms larval homogenate of Ascaridia galli diluted in 1ml PBS and emulsified in 1ml Freund’s adjuvant orally. Booster immunization was also administered orally on day 21 The other group (5 white leghorn hens) received 1 ml PBS emulsified in 1ml of the same adjuvant. On day 33 each hen was administered approximately 10,000 Ascaridia galli infective eggs orally. Blood samples were collected from the animals in a 10 day interval from the first immunization until infective challenge and weekly thereafter until the end of study. Sera were tested by ELISA and Western-blotting. Hens were necropsied 8 weeks post challenge with infective eggs for recovery of the nematodes. Vaccinated hens showed a 76 percent reduction in mean EPG, 74 percent reduction in the average number of male worms, 79 percent reduction in the average number of female worms and 77 percent reduction in the mean number of total worms. Significance in mean optical density of sera in ELISA was noticed (p<0.001). Proteins of Ascaridia galli were separated by SDS-PAGE. The vaccinated sera indicated a high reactivity in Western-Blotting.

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