Bovine Leukemia Virus DNA detection in Pasteurized Milk from some Dairy Industries Company in Iran

Subject Areas : Veterinary Clinical Pathology

Shobeir Yazdani Paraei ¹ , mohammad rahim hajikolayei ² , masoudreza Siefi abad shapori ³ , Mohammad Nouri ⁴ , Faramarz Beheshtifar ⁵

1 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 - استاد ﮔﺮوه ﻋﻠﻮم درﻣﺎﻧﮕﺎﻫﻲ، داﻧﺸﻜﺪه داﻣﭙﺰﺷﻜﻲ، داﻧﺸﮕﺎه ﺷﻬﻴﺪ چمران اهواز، اهواز، ایران.
3 - Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz ,Iran.
4 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
5 - Department of Surgery, School of medicine, Ahvaz Jundishpur University of Medical sciences

Received: 2024-01-15 Accepted : 2025-01-08 Published : 2025-03-03

Keywords: Bovine leukemia virus, Enzootic bovine leukosis, Pasteurized milk, Iran,

Abstract :

Bovine leukemia virus (BLV) as the causative agent of enzootic bovine leukemia (EBL) is an RNA virus that closely related to human T-cell leukemia virus type 1 and type 2 and probably known the cause of breast cancer in women. The virus transmits to human through animal products such as milk and meat. This study aimed to detect DNA of Bovine leukemia virus in pasteurized milk. For this purpose, the samples of pasteurized milk were taken from 5 Dairy Industries Company in Tehran, Mazandaran, Khouzestan and Farse provinces of Iran. The samples were taken from the pasteurized milk available in Ahvaz city stores, so that 10 milk samples with different production dates were taken from each factory and stored at -20 centigrade until examination. They were examined by nested PCR to detect Bovine leukemia virus DNA. Out of 50 samples of pasteurized milk from different factories, 14(28%) samples were positive. Statistical analysis showed there was no significant difference between the milk produced by different factories studied and also between factories in two factory of Tehran with other provinces. The results of this study show that this virus is present in pasteurized milk, but whether it is active or not requires further investigation.