Combination effect of swimming exercise and genistein treatment on the IL1 β factor in the lung of ovariectomized diabetic rats

Subject Areas : Veterinary Clinical Pathology

Masoumeh Majidi zolbin ¹ , Faeze Daghigh ²

1 - Assistant Professor, Pediatric Urology and Regenerative Medicine Research Center, Gene, Cell and Tissue Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
2 - Assistant Professor, Department of Physiology, Faculty of Medical Sciences, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran.

Received: 2024-01-17 Accepted : 2024-06-22 Published : 2024-11-20

Keywords: Lung, Ovariectomy, IL1&beta, , Diabetes,

Abstract :

Menopause and diabetes are associated with significant increase in the expression of inflammatory markers in respiratory system. In the present study, we investigated the simultaneous effect of swimming exercise and treatment with genistein on the lungs of ovariectomized diabetic rats. In this experiment, sixty female Wistar rats were randomly divided into six groups. Healthy control: (sham), ovariectomized female rats: (OVX) (rats that underwent bilateral ovariectomy), ovariectomized diabetic group: (OVX.Dia) (ovariectomized rats that were fed with high-fat diet), ovariectomized diabetic group treated with swimming: (OVX.Dia. Exe) (8 weeks of swimming training), ovariectomized diabetic group treated with genistein: (OVX.Dia. Gen) (8 weeks genistein administration, 1 mg/kg /day, s.c.), ovariectomized diabetic group treated with genistein and swimming exercise: (OVX.Dia. Gen.Exe). To induce diabetes, the diabetic groups received a high-fat diet for four weeks. The studied groups did swimming exercise or received genistein for eight weeks. At the end of the experiment, the lung tissue was removed under anesthesia and the amount of IL1β factor in the lung tissue was analyzed by western blot technique. The amount of IL1β factor in ovariectomized and ovariectomized diabetic rat was significantly increased compared to the control group. Genistein treatment and swimming exercise in ovariectomized diabetic rats decreased the amount of IL1β factor in the studied groups compared to the ovariectomized diabetic group. Swimming training and genistein treatment ameliorated expression levels of inflammatory biomarkers (IL1β) in the lung of ovariectomized diabetic rats.

References:

• Alipour, M.R., Ahmadiasl, N., Karimi, P., Alihemmati, A., Zolbin, M.M. and Daghigh, F. )2018(. Effects of genistein treatment on molecular markers related to fibrosis: addition to histological changes in the lung of ovariectomized diabetic rats. Journal of Contemporary Medical Sciences, 4(3): 158-162.
• Belotto, M.F.J., Magdalon, H.G., Rodrigues, M.A., Vinolo, R., Curi, T., Pithon-Curi, C., et al. (2010). Moderate exercise improves leucocyte function and decreases inflammation in diabetes. Clinical Experimental Immunology, 162(2): 237-243.
• Bitoska, I., Krstevska, T., Milenkovic, S., Subeska-Stratrova, G., Petrovski, S.J., Mishevska, I., et al. (2016). Effects of Hormone Replacement Therapy on Insulin Resistance in Postmenopausal Diabetic Women. Open Access Macedonian Journal of Medical Sciences, 4(1): 83-88.
• Daghigh, F., Alihemmati, A., Karimi, P., Habibi, P. and Ahmadiasl, N. (2017). Genistein preserves the lungs of ovariectomized diabetic rats: addition to apoptotic and inflammatory markers in the lung. Iranian Journal of Basic Medical Sciences, 20(12): 1312-1317.
• Daghigh, F., Alihemmati, A., Karimi, P., Habibi, P. and Ahmadiasl, N. (2018). Fibrotic and apoptotic markers alteration in ovariectomised rats: addition of swimming training preserves lung architecture. Archives of Physiology abd Biochemistry, 124(4): 286-291.
• Dennis, J., Maldonado, D., Rojas, X., Aschner, P., Rondón, M., Charry, L., et al. (2010). Inadequate glucose control in type 2 diabetes is associated with impaired lung function and systemic inflammation: a cross-sectional study. BMC Pulmonary Medicine, 10: 38.
• Dogru, Z., Nacar, T., Unal, D., Aksak, S., Albayrak, A., Odabasoglu, F., et al. (2012). Effects of diabetes mellitus and postmenopausal period on the lungs of rats. African Journal of Pharmacy and Pharmacology, 6(27): 1989-2010.
• Fan, H., Wang, Y., Zhao, K., Su, L., Deng, C., Huang, J., et al. (2023). Incomplete Knockdown of MyD88 Inhibits LPS-Induced Lung Injury and Lung Fibrosis in a Mouse Model. Inflammation, 46(6): 2276-2288.
• Glassberg, K., Choi, R., Manzoli, V., Shahzeidi, S., Rauschkolb, P., Voswinckel, R., et al. (2014). 17β-estradiol replacement reverses age-related lung disease in estrogen-deficient C57BL/6J mice. Endocrinology, 155(2): 441-448.
• Habibi, P., Alihemmati, A., NourAzar, A., Yousefi, H., Mortazavi, S. and Ahmadiasl, N. (2016). Expression of the Mir-133 and Bcl-2 could be affected by swimming training in the heart of ovariectomized rats. Iranian Journal of Basic Medical Sciences, 19(4): 381-387.
• Kim, S., Lee, H., Kim, C., Ko, H., Yoon, S. and Yi, K. (2014). Effect of exercise training of different intensities on anti-inflammatory reaction in streptozotocin-induced diabetic rats. Biology Sport, 31(1): 73-79.
• Kim, J. and Lim, Y. (2013). Protective effect of short-term genistein supplementation on the early stage in diabetes-induced renal damage. Mediators of Inflammation, 2013: 510212.
• Massaro, D. and Massaro, G. (2004). Estrogen regulates pulmonary alveolar formation, loss, and regeneration in mice. American Journal of Physiology-Lung Cellular and Molecular Physiology, 287(6): L1154-1159.
• Mohajeri, D., Mesgari, M., Rezaie, A. and Balila, A. (2007). Histopathological study of osteoporotic changes after experimental creation of osteoporosis by ovariectomy in the rat. Veterinary Clinical Pathology The Quarterly Scientific Journal, 1(2): 97-108.
• Mosca, L., Collins, M., Herrington, E., Mendelsohn, C., Pasternak, M., Robertson, K., et al. (2001). Hormone replacement therapy and cardiovascular disease: a statement for healthcare professionals from the American Heart Association. Circulation, 104(4): 499-503.
• Nakhaei, H., Mogharnasi, M. and Fanaei, H. (2019). Effect of swimming training on levels of asprosin, lipid profile, glucose and insulin resistance in rats with metabolic syndrome. Obesity Medicine, 15: 100111.
• Oh, Y., Lim, S., Lee, M., Kim, Y., Ann, S. and Yoon, S. (2007). A combination of soy isoflavone supplementation and exercise improves lipid profiles and protects antioxidant defense-systems against exercise-induced oxidative stress in ovariectomized rats. Biofactors, 29(4): 175-185.
• Ortega-Santos, P., Al-Nakkash, L. and Whisner, M. (2020). Exercise and/or Genistein Treatment Impact Gut Microbiota and Inflammation after 12 Weeks on a High-Fat, High-Sugar Diet in C57BL/6 Mice. Nutrients, 12(11).
• Quinteiro, H., Buzin, M., Conti, F., Dias Dda, S., Figueroa, D., Llesuy, S., et al. (2015). Aerobic exercise training promotes additional cardiac benefits better than resistance exercise training in postmenopausal rats with diabetes. Menopause, 22(5): 534-541.
• Sadeghian, R., Shahidi, S., Komaki, A., Habibi, P., Ahmadiasl, N., Yousefi, H., et al. (2021). Synergism effect of swimming exercise and genistein on the inflammation, oxidative stress, and VEGF expression in the retina of diabetic-ovariectomized rats. Life Sciences, 284: 119931.
• Silva, M., Duarte-Mendes, P., Teixeira, M., Soares, M. and Ferreira, P. (2024). The effects of combined exercise training on glucose metabolism and inflammatory markers in sedentary adults: a systematic review and meta-analysis. Science Report, 14(1): 1936.
• Stephenson, J., Setchell, D., Kendall, W., Jenkins, J., Anderson, W. and Fanti, P. (2005). Effect of soy protein-rich diet on renal function in young adults with insulin-dependent diabetes mellitus. Clinical Nephrology, 64(1):1-11.
• Su, X., Meng, X., Sun, c., Liu, l. and Su, B. (2011). Intramuscular injection of soluble receptor for advanced glycation endproducts expression vector prevents the development of streptozotocin-induced diabetes mellitus in rats on high fat diet. Journal of Diabetes, 3(4): 309-316.
• Witayavanitkul, N., Werawatganon, D., Chayanupatkul, M., Klaikeaw, N., Sanguanrungsirikul, S. and Siriviriyakul, P. (2020). Genistein and exercise modulated lipid peroxidation and improved steatohepatitis in ovariectomized rats. BMC Complementary Medical Therapy, 20(1): 162.
• Yeh, C., Punjabi, M., Wang, Y., Pankow, S., Duncan, B., Cox, E., et al. (2008). Cross-sectional and prospective study of lung function in adults with type 2 diabetes: the Atherosclerosis Risk in Communities (ARIC) study. Diabetes Care, 31(4): 741-746.
• Zhang, N., Zhang, W., Guo, X., Liu, J., Li, S., Zhang, H., et al. (2022). Genistein protects against hyperglycemia and fatty liver disease in diet-induced prediabetes mice via activating hepatic insulin signaling pathway. Frontier in Nutrition, 9: 1072044.