Therapeutic effects of Vitex (Vitagnus castus) extract on in vitro maturation and fertilization of oocytes in mice affected by polycystic ovary syndrome

Subject Areas : Veterinary Clinical Pathology

tohid  ghorbani ¹ (MSc Student, Department of Animal Science, Faculty of Ahar Agriculture and Natural Resources, University of Tabriz, Tabriz, Iran.)
amir  karimi ² (Assistant Professor, Department of Animal Science, Faculty of Ahar Agriculture and Natural Resources, University of Tabriz, Tabriz, Iran.)
gholamreza  najafi ³ (Associated Professor, Department of Anatomy, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.)
maghsud  besharti ⁴ (Assistant Professor, Department of Animal Science, Faculty of Ahar Agriculture and Natural Resources, University of Tabriz, Tabriz, Iran.)
Mouhsen  Sharafi ⁵ (Assistant Professor, Department of Poultry Sciences, Faculty of Agriculture, Tarbiat Modarres University, Tehran, Iran.)

Received: 2019-11-09 Accepted : 2020-09-05 Published : 2020-08-22

Keywords: in vitro fertilization, Vitex, PCOs, In vitro maturation,

Abstract :

Polycystic ovary syndrome results from lack of follicular wall collagen reduction leading to accumulation of follicles and decreased oocyte quality. This study aimed to evaluate the effect of vitex extract on in vitro maturation and fertilization of oocytes in mice exposed to polycystic ovary syndrome (PCOs). A total of 32 immature Naval Medical Research Institute (NMRI) 25 day old female mice with a mean body weight of 25 gr were randomly allocated into four experimental groups including: 1) Control group (Con) which did not receive extract, 2) PCO: polycystic ovary syndrome group which also did not receive any extract, and groups 3 and 4 in which PCO was induced and received vitex extract at 365 and 730 mg/kg/day respectively for 30 days.  The mice received intraperitoneal injection of pregnant mare serum gonadotropin (PMSG) and subsequently sacrificed in order to collect oocytes from ovarian follicles for in vitro analysis of maturation and fertilization. The amounts of serum testosterone and estradiol substantially decreased in extract treated groups in comparison with PCO mice (p < /em><0.05). Use of vitex extract decreased testosterone whether in groups PCO+365 mg of extract or PCO+730 mg of extract versus PCO (p < /em><0.05). The number of mature oocytes (MII) in PCO+730 mg of extract was significantly higher than PCO (p < /em><0.05). Also there were no significant differences in percentage of fertilized oocytes and Two-cell embryos between experimental groups (p < /em>>0.05). On the other hand, the percentage of produced blastocyst and hatched embryos in PCO+365 mg of extract was higher than PCO (p < /em><0.05). The results showed vitex extract consumption can induce more oocytes for fertilization and consequently, production of embryos in animals with PCO syndrome.

References:

• Altonen, J., Laitinen, M.P., Voujolainen, K., Jaatinen, R., Horelli-­Kuitunen, N. and Seppä, L. (1999). Human growth differentiation factor 9 (GDF-9) and its novel homolog GDF-9B are expressed in oocytes during early folliculogenesis. The Journal of Clinical Endocrinology and Metabolism, 84(8): 2744-2750.


• Borrione, P., Di Luigi, L., Maffulli, N. and Pigozzi, F. (2008). Herbal supplements: cause for concern? Journal of Sport Science and Medicine, 7(4): 562-564.


• Brock, B., Smidt, K., Ovesen, P., Schmitz, O. and Rungby, J. (2005). Is metformin therapy for Polycystic Ovary Syndrome safe during pregnancy? Basic & Clinical Pharmacology and Toxicology, 96(6): 410-412.


• Daghigh Kia, H., Sadeghi Sadegh Abad, F., Ebrahimi, M. and Samadian, F. (2017). Comparative effect of different concentrations of hydro-ethanolic extract of chamomile on freeze-thawn semen quality of rams. Veterinary Clinical Pathology, 11(41): 13-23. [In Persian]


• Daniele, C., Thopson, C.J., Pittler, M.H. and Ernest, E. (2005). Vitex agnus-­castus a systemathic review of adverse event. Drug Safety, 28(4): 319-322.


• Dumesic, D.A., Meldrum, D.R., Katz-Jaffe, M.G., Krisher, R.L. and Schoolcraft, W.B. (2015). Oocyte environment: follicular fluid and cumulus cells are critical for oocyte health. Fertility and Sterility, 103(2): 303-316.


• Dumesic, D.A, Padmanabhan, V. and Abbott, D.H. (2008). Polycystic ovary syndrome and oocyte developmental competence. Obstetrics and Gynecology, 63(1): 39-48.


• Franks, S. (2009). Do Animal Models of Polycystic Ovary Syndrome Help to Understand Its Pathogenesis and Management? Yes, but Their Limitations should be Recognized. Endocrinology, 150(9): 3983-3985.


• Gonzalez, F., Thusu, K., Abdel-Rahman, E., Prabhala, A., Tomani, M. and Dandona, P. (1999). Elevated serum levels of tumor necrosis factor alpha in normal-weight women with polycystic ovary syndrome. Metabolism, 48(4): 437-441.


• Henmi, H., Endo, T., Nagasawa, K., Hayashi, T., Chida, M. and Akutagawa, N. (2001). Lysyl oxidase and MMP-2 expression in dehydroepiandrosterone induced polycystic ovary in rats. Biology of Reproduction, 64(1): 157-162.


• Hossain Rashidi, B. and Nemati, M. (2017). Effects of Vitex agnus-castus extract on the secretory function of pituitary-gonadal axis and pregnancy rate in patients with premature ovarian aging (POA). Journal of Herbal Medicine, 10: 24-30.


• Handa, R.J., Pak, T.R., Kudwa, A.E., Lund, T.D. and Hinds, L. (2008) An alternate pathway for androgen regulation of brain function: activation of estrogen receptor beta by the metabolite of dihydrotestosterone, 5alpha-androstane-3beta,17 beta-diol. Hormones and Behavior, 53(5): ­741-752.


• Jelodar, G.A. and Askari, K. (2012). Effect of Vitex agnus castus fruits hydroalcoholic extract on sex hormones in rat with induced polycystic ovary syndrome (PCOS). Journal of Physiology and Pharmacology, 16(1): 62-69. [In Persian]


• Jelodar, G.A. and Askari, K. (2017). Effect of hydroalcoholic extract of Vitex agnus-castus fruit on fertility and estrous cycle in letrozole-­induced polycystic ovary syndrome in rat. Razi Journal of Medical Sciences, 24(156): 42-48. [In Persian]


• Marx, T.L. and Mehta, A.E. (2003). Polycystic ovary syndrome: pathogenesis and treatment over the short and long term. Cleveland Clinic Journal of Medicine, 70(1): 31-45.


• Mohammadzadeh, H., Delashoub, M. and Khakpour, M. (2018). Effect of vitamin E in prevention of lipopolysaccharide induced fetal injuries in the rat. Veterinary Clinical Pathology, 11(44): 367-377. [In Persian]


• Mostahsan, Z. and Mortazavi, P. (2019). The effects of Tilia (Tilia plathyphyllos) extract on gene expression of caspase­3 and caspase­9 in canine mammary gland cancer cell line (CF41.Mg). Veterinary Clinical Pathology, 13(49): 2-14. [In Persian]


• Nabiuni, M., Mohammadi, S., Kayedpoor, P. and Karimzadeh, L., (2015). The effect of curcumin on the estradiol valerate-induced polycystic ovary in rats. Kashan University of Medical Sciences Journal (FEYZ), 18(6): 515-523. [In Persian]


• Nasri, S., Oryan, S.h., HaeriRohani, A., Amin, G.H. and Taghizadeh, M. (2005). The effects of Vitexagnuscastus L. extract and interaction with bromocriptine on luteinizing hormone and testosterone in male mice. Medical Journal of Hormozgan University, 9(2): 113-118. [In Persian]


• Noorafshan, A., Ahmadi, M., Mesbah, S. and Karbalay-Doust, S. (2013). Stereological study of the effect of letrozole and estradiol valerate treatment on the ovary of rats. Clinical and Experimental Reproductive Medicine, 40(3): 115-121.


• Palep-Singh, M., Picton, H.M., Yates, Z.R., Barth, J.H. and Balen, A.H. (2008). Plasma homocysteine concentrations and the single nucleotide polymorphisms in the methionine synthase gene (MTR 2756A>G): Associations with the polycystic ovary syndrome an observational study. The European Journal of Obstetrics & Gynecology and Reproductive Biology, 138(2): 180-186.


• Rani, A. and Sharma, A. (2013). The genus Vitex: A review. Pharmacognosy Reviews, 7(14): 188–198.


• Robinson, J.E., Forsdike, R.A. and Taylor, J.A. (1999). In utero exposure of female lambs to testosterone reduces the sensitivity of the GnRH neuronal network to inhibition by progesterone. Endocrinology, 140(12): 5797-5805.


• Shafiee, M.N., Malik, D.A., Yunos, R.I.M., Atiomo, W., Omar, M.H., Ghani, N.A.A. et al. (2015). The effect of Metformin on endometrial tumor-regulatory genes and systemic metabolic parameters in polycystic ovarian syndrome–a proof-of-concept study. Gynecological Endocrinology, 31(4): 286-290.


• Shamsi, M., Nejati, V. and Najafi, G. (2015). Therapeutic Effects of Licorice Extract on In vitro Maturation and In vitro Fertilization in Mice Model of Polycystic Ovary Syndrome. Journal of Mazandaran University of Medical Science, 25(132): 113-121. [In Persian]


• Stadtmauer, L.A., Toma, S.K., Riehl, R.M. and Talbert, L.M. (2001). Metformin treatment of patients with polycystic ovary syndrome undergoing in vitro fertilization improves outcomes and is associated with modulation of the insulin-like growth factor. Fertility and Sterility, 75(3): 505-509.


• Tsilchorozidou, T., Overton, C. and Conway, G.S. (2004). The pathophysiology of polycystic ovary syndrome. Clinical Endocrinology, 60(1): 1-17.


• van Houten, E.L. and Visser, J.A. (2014). Mouse models to study polycystic ovary syndrome: a possible link between metabolism and ovarian function? Reproductive Biology, 14(1): 32-43.