Effects of Chamaemelum nobile hydro alcoholic extract on reproductive and behavioural parameters in male rats exposed to immobility stress

Subject Areas : Veterinary Clinical Pathology

Mehrdad  Neshat Gharamaleky ¹ (Assistant Professor, Department of Clinical Science, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.)
yasin  bagheri ² (Young Researchers and Elite Club, Tabriz Branch, Islamic Azad University, Tabriz, Iran.)
masoud  delashoub ³ (Assistant Professor, Department of Basic Science, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.)
hesam  rafie ⁴ (Young Researchers and Elite Club, Tabriz Branch, Islamic Azad University, Tabriz, Iran.)
Ali mohammad  Bahrami ⁵ (Associate Professor, Department of Pathobiology, Para Veterinary Faculty of Ilam, University of Ilam, Ilam, Iran.)
Aref  Delkhosh ⁶ (D.V.M. Graduate, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.)

Received: 2019-09-30 Accepted : 2020-05-10 Published : 2020-04-20

Keywords: sperm, Rat, Anthemis nobilis L, immobility stress,

Abstract :

Stress is a very important factor in causing disorders in the reproductive system. Considering the effects of Anthemis nobilis L. in traditional medicine as an effective treatment of certain diseases, the aim of this study was to evaluate the effect of hydro-alcoholic extract of Anthemis nobilis L.onreproductive and behavioural parameters in male rats exposed to immobility stress. Thirty male Wistar rats weighing 220±20 g were randomly divided into five groups including control without any stress and additive, control exposed to stress without additive (positive control) and treatment groups exposed to stress and receiving Anthemis nobilis L.extract in dosages of 50, 100 and 200mg/kg respectively. The extract was given to the rats with gavage and accompanied by immobility stress for 21 days. At the end of the study, anxiety was evaluated using elevated plus maze, also blood samples were collected for hormonal assay and the number of sperms were assessed. Obtained data were analyzed using one way ANOVA followed by Tukey post hoc test. Hydro-alcoholic extract of Anthemis nobilis L.significantly increased the number of sperms, testosterone, LH and FSH levels in comparison to the stress group (p < /em><0.05). Also the findings of this research revealed that the extract led to significant increase in percentages of open arm entry and decrease in percentages of close arm entries in the elevated plus maze compared to the stress group (p < /em><0.05). The results indicated that hydro alcoholic extract of Anthemis nobilis L.has anxiolytic effects and increases the reproductive parameters probably due to antioxidant components of this plant.

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