The effects of pomegranate skin extract and powder on renal tissue structure following experimental cadmium poisoning in Japanese quail

Subject Areas : Veterinary Clinical Pathology

Rahmat Allah Fatahian Dehkordi ¹ , Mohammad Reza Alijani ² , rasool rahimi Junqani ³ , Shahab Bahadoran ⁴

1 - Associate Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
2 - Student of Veterinary Medicine, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
3 - Student of Veterinary Medicine, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
4 - Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.

Received: 2019-05-01 Accepted : 2020-02-16 Published : 2020-04-20

Keywords: Cadmium, Japanese quail, kidney, pomegranate, Lipid profile,

Abstract :

Chronic exposure to cadmium, a trace metal, leads to poisoning in humans and animals. The purpose of this study was to investigate the effects of pomegranate skin extract and powder on tissue structure of the kidneys following experimental cadmium poisoning in Japanese quail. For this purpose, 510 seven day old Japanese quail chicks were divided into 10 groups. Groups 1 and 2 served as negative control and cadmium poisoning control (20 ppm) respectively, groups 3 and 4 received 0.1% and 0.2% pomegranate skin extract along with 20 ppm of cadmium respectively, groups 5 and 6 received 1% and 2% pomegranate skin powder along with 20 ppm of cadmium respectively, groups 7 and 8 received 0.1% and 0.2% pomegranate skin extract respectively and groups 9 and 10 received 1% and 2% pomegranate skin powder respectively. On day 42, all birds were slaughtered and kidney samples were removed from the abdominal area and transferred to formalin, then 5 micrometer sections were prepared and stained and a stereological study was performed using a point grid. The results showed no significant difference between the groups in the number of distal tubules and the area of the interstitial tissue. However, significant differences were observed in the number of proximal tubules between groups 9 and 2. Pathologic findings also revealed tissue damages in treatment groups which had received cadmium. Cholesterol levels were significantly decreased in groups which had received 0.2% and 0.1% pomegranate skin extract along with cadmium in comparison to cadmium control group. It can be concluded that the use of pomegranate skin extract and powder does somewhat improve recovery conditions in the kidney structure after administration of cadmium.

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