Effect of cations on sperm motility and fertilization capacity in Grass carp (Ctenopharyngodon idella)

Subject Areas : New Technologies in Aquaculture Development

M.  Ahmadnejad ¹ (1 Inland Water Aquaculture Research Center, Bandar Anzali, Iran)

Received: 2021-10-17 Accepted : 2021-10-17 Published : 2021-08-23

Keywords: sperm, hatching, Ions, Grass carp, Keywords: Fertilization,

Abstract :

 Abstract[1]Saline solutions containing cations (Na+, K+, Ca+2, Mg+2) were used to investigate the effect of ions on motility characteristics of spermatozoa (sperm movement duration and percentage of motile spermatozoa) and frtilizing capacity of sperm (fertilization rate, hatching rate, larvae length during hatching, larvae length during active feeding and survival rate) in Ctenopharyngodon idella. To survey of the effects of ions on sperm motility, Na+ (NaCl) (2208, 2393 and 2576 mg/L), K+ (KCl) (1200, 1400 and 1800 mg/L), Mg2+ (MgCl2) (132, 228 and 324 mg/L) and Ca2+ (CaCl2) (1000, 1200 and 1400 mg/L) were used. The results showed that, the highest duration of sperm motility and fertilization rate were obtained in solutions containing 2393 and 2576 ml-1 NaCl (P<0.05), respectively. Solutions containing different doses of KCl caused a decrease in percentage of motile spermatozoa, fertilization and hatching rates (P<0.05). The duration of sperm motility decreased in solution containing MgCl2 and immediately after activating by 1200 mgl-1 CaCl2 motility was ceased. Highest hatching rate (80±5 mg/L), survival rate (92.29±0.91 mg/L) were observed in solution containing 1000 mgl-1 CaCl2. With regard to our results can be conclude that NaCl and CaCl2 are influencing factors involved in fertilizing capacity of grass carp. * Corresponding Authors; Email: [e-mail]