Effect of Seed Priming with Sodium-Ascorbate on Germination and some of Physiological Characteristics of Sesame (Seamum indicum) under Different Temperatures

Subject Areas : Journal of Crop Ecophysiology

P. Tizfahm ¹ ( M.Sc. of Agronomy, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran )
A. Ebadi ² ( Associate Prof., Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran )
H. Tavakoli ³ ( Ph.D. Student of Crop Physiology, Faculty of Agricultural Sciences, University of Mohaghegh Ardabili, Ardabil, Iran )
N. Tavakoli ⁴ ( Ph.D. Student of Crop Physiology, Faculty of Agricultural Sciences, University of Mohaghegh Ardabili, Ardabil, Iran )

Received: 2014-10-15 Accepted : 2015-11-11 Published : 2015-12-22

Keywords: Protein, Sesame, peroxidase, Sodium Ascorbate,

Abstract :

To study the effects of ascorbate and temperature on antioxidant enzymes and germination indexes, a factorial experimental based on completely randomized block design with three replications was conducted at the Agricultural Research Laboratory of Mohaghegh Ardabili University in 2014. Treatments were three levels of ascorbate (control, 5 and 10 mM) and three levels of temperatures (15, 25 and 35 °C). Parameters under study were germination indices, production rates of antioxidant enzymes, proline and protein. The results of experiment indicated that ascorbate application that of raised proline content. Application of ascorbate resulted in enzymes reduction at 15°C more than 25°and 35° temperatures. Its application also reduced protein denaturation. Since sesame is a warm season plant, it seems that increasing antioxidant enzymes at 15°C is due to higher production of free radicals. The positive and significant correlation between antioxidant enzymes and germination indices represents their direct relationships. According to the results of this experiment, it seems that the application of ascorbate had positive effect on germination indices and reduced the effects of improper temperatures; it also benefited seedling growth of sesame.

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