Production of bioplastics by bacilli isolated from the landfill of waste and sewage in Golestan province

Subject Areas : microbiology

Amin Lotfvarzi ¹ , seyed Taleb houseini ² , Ali Bagheri hashem abad ³ , Hamid reza Pordeli ⁴ , Farhad Yolmeh ⁵ , Ania Ahani azari ⁶

1 - Department of Biology, Faculty of Basic Sciences, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran
2 - Department of Biology, Faculty of Basic Sciences, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran
3 - Department of Biology, Faculty of Basic Sciences, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran
4 - Department of Biology, Faculty of Basic Sciences, Gorgan Branch, Islamic Azad University, Gorgan, Iran
5 - Department of Biology, Faculty of Basic Sciences, Gorgan Branch, Islamic Azad University, Gorgan, Iran
6 - Department of Biology, Faculty of Basic Sciences, Gorgan Branch, Islamic Azad University, Gorgan, Iran

Received: 2021-10-07 Accepted : 2021-10-21 Published : 2021-05-22

Keywords: Bacillus, Bioplastic, Polyhydroxy butyrate, Polyhydroxy Alkanoate,

Abstract :

Millions of tons of plastics are produced annually in the world, which are very durable and take hundreds of years to decompose on their own. Bioplastics are a special type of plastic that is produced from sources of vegetable oils, starches, cellulose or bacterial derivatives and is very different from ordinary plastics in this regard, which will be a good alternative to plastics in the future. 70 samples were collected from waste recycling site, cultured and purified on nutrient agar and these samples were stained with blue indigo staining method to detect polyhydroxy butyrate (PHB) generating isolates And cultured for 2 days at 37 ° C in Minimal Broth Davis medium.After placing the samples on the slide and staining according to the intensity of the color that was created, it was used to produce bioplastics. The results of this study showed that the microorganism Bacillus cereus produces abundant poly-3-hydroxybutyrate in both environments (wastewater and soil), which is used to produce bioplastics on both laboratory and industrial scales. According to the results, the production of bioplastics is possible through bacilli isolated from bacterial isolates.