In vitro of essential oils effects on mycellial growth of Fusarium solani the causal of storage disease Fusarium Dry Rot of potato on their tubers.

Subject Areas : Plant Pests

Roya Tavousi ¹ , Mohammad Torabi ² , Hosain Vafaei ³

1 - Departement of Plant Pathology, College of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran.
2 - Departement of Plant Pathology, College of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
3 - Agriculthral and Natural Resources Research Center of Khoramabad, Khoramabad, Iran.

Received: 2014-09-16 Accepted : 2015-01-10 Published : 2014-12-22

Keywords: Essential oils, F. solani, Antifungal Compounds, Fungal Storage Inhibition of Mycelia Growth, Rot Control on potato tuber,

Abstract :

The use of antifungal compounds such as essential oils for the preservation of natural fruits during the storage period has dramatically been rising. In this study, the individual essential oils of Majorom (Origamum marjorana), wild mint (Mentha longifolia) and Summer savory (Satureja khuzistanica) were employed for the control of corruption of tubers during the storage period. In this study, the antifungal activity of essential oils was investigated on growth a specie of plant pathogenic fungus (Fusarium solani)on PDA medium and tuber tissues.  Antifungal activity on PDA medium were measured by disk diffusion and volatile essential oil methods. To evaluate the effects of the essential oils on fungal growth in tuber tissue, the potato were inoculated with 30 µl of spore suspension (106 spore/ml), and then tubers were sprayed with 4 and 20 µl/ml of the essential oils. Seven days after inoculation, tubers were evaluated in terms of pollution. To calculate the percentage of infected tuber was divided into eight parts and the number of infected tuber fungus pathogen contamination in 12.5% multiplication and percentages were calculated. Data were analyzed as a completely randomized design with three replications. Essential oils has showed Significant inhibitory effect on fungus growth. The results showed that most inhibition of growth in a paper disk method was in order for fungus F. solani related to the essential oil of summer savory 59.41 percent, respectively and volatile essential oil methods was related to the essential oil of Summer savory was 41.12 percent respectively. The results inoculated of the tuber tissue with a spore suspension F. solani showed that respectively treatment (20µl/ml) of Majorom (with 27.08% of disease) had the most inhibitory against pathogen