Structural, and phylogenetic analysis of cytochrome P450 protein in insects
Subject Areas : Plant Pests
1 - Assistant Professor, Department of Plant Pathology, College of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran.
Keywords: Structural analysis, phylogenetic tree, Bioinformatics, cytochrome P450 (cyp), Insects,
Abstract :
Cytochrome P450 (cyp) enzymes are heme-containing monooxygenases that catalyze metabolisms of various endogenous and exogenous compounds. They constitute a superfamily of enzymes present in various organisms including mammals, plants, bacteria, and insects. cyps are diverse and metabolize a wide variety of substrates, but their structures are largely conserved. In this study, bioinformatic analyses of P450 enzymes were performed in 30 insect species from different families. According to the conserved motifs obtained by MEME and MAST tools, three motifs were common in all insect species. The structural analyses of seven selected species of insects were investigated by ProtParam and SOPMA tools in ExPASy database. Tertiary structure of Drosophila melanogaster (Accession code: NP_525031) as an insect sample and six samples of other species were predicted by Phyre2 server using “c4lxjA” model (PDB accession code: 4lxj). The protein sequences were aligned with ClustalW algorithm by MEGA 6.06, and phylogenetic tree was constructed using the Neighbor-joining (NJ) method. According to the results, there is a high similarity among cyp proteins of different species in insects so that they should be derived from a common ancestor. The obtained data provides background of bioinformatic studies for the function and evolution of this protein in insects.
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