Identification of resistant genes against blast in genotypes cultivated in north of Iran using molecular markers

 

 

Subject Areas : Plant Pathology

Maryam Farifteh ¹ (Plant Protection Department, Agriculture Faculty, Islamic Azad University Science and Research Branch, Tehran, Iran)
Bahar Morid ² (Plant Protection Department, Agriculture Faculty, Islamic Azad University Takestan Branch, Takestan, Qazvin, Iran)
Hamidreza Zamanizadeh ³ (Plant Protection Department, Agriculture Faculty, Islamic Azad University Science and Research Branch, Tehran, Iran)

Received: 2023-05-02 Accepted : 2023-07-11 Published : 2024-02-06

Keywords: Rice Blast Disease, Resistance gene, Pia, Pib, Pi5 ,

Abstract :

Blast disease caused by Magnaporthe grisea is a significant threat to rice worldwide, leading to substantial damage and yield losses. The most effective method for controlling this disease is through the use of resistant rice cultivars that carry specific resistance genes. In this study, three primers (Yca7, Nsb, and JJ718) were used associated with the Pia, Pib, and Pi5 resistance genes, respectively, to identify blast-resistant rice cultivars and lines. At first, DNA was extracted from the leaves of 10-day-old seedlings of the entered rice cultivars and lines using the CTAB method. Subsequently, polymerase chain reaction (PCR) and electrophoresis were conducted to amplify and analyze the resulting DNA bands with the respective primers. Our findings indicated that Qaim and Gilane cultivars carry the Pia resistance gene, making them probable candidates for rice blast disease resistance. Similarly, all tested cultivars (excluding Sahel) possess the Pib resistance gene, suggesting their resistance to blast disease. Furthermore, except for Sepidrood and Sahel cultivars, all cultivars tested positive for the Pi5 resistance gene, indicating their ability to resist blast disease. Based on these results, Qaim and Gilane cultivars exhibit resistance to the disease as they carry all three resistance genes, while Sahel cultivar lacks all three resistance genes, making it the most susceptible cultivar in our study.

References:

حسینی ایمنی، ص. ۱۳۸۲. بررسی اثر تاریخ نشاکاری، فواصل بوته و کود ازته بر شاخص های رشد، عملکرد و اجزاء عملکرد لاین جدید برنج ۸۰۰۸. پایان نامه کارشناسی ارشد زراعت. دانشگاه مازندران.
زربافی، س.، ربیعی، ب، و عبادی، ع. ا. 1398. غربال ژنوتيپ¬هاي برنج (Oryza sativa L.) براي تحمل و حساسيت به بلاست برگ تحت شرايط آلودگي مصنوعي در مزرعه. تحقیقات غلات 9(3): 193-206.
عابدی، ف.، بابائیان، ن. د.، مؤمنی، ع. و نعمت¬زاده، ق. ع. 1390. بررسی مقاومت نسبی گیاهچه¬های برخی ارقام برنج نسبت به عامل بلاست (Magnaporthe grisea (Hebert) Barr) در شرایط مزرعه و گلخانه. مجله دانش زراعت 2(4): 42-31.
فهیمی¬فر، ج. 1370. بازار جهانی برنج. انتشارات موسسه مطالعات و پژوهش های بازرگانی. 505 صفحه.
مرادی، ز.، سالاری، م.، رمضانی، م، مؤمنی، ع. و موسی¬نژاد، ص. 1388. تجزیه ژنتیکی مقاومت برنج به بلاست برگ با استفاده از طرح دای‌آلل. دانش گیاهپزشکی ایران 40(2): 109-116.
موسي¬نژاد، ص.، مومني، ع. و نيك خواه م. ج. 1389. ارزیابی اجزای مقاومت به بیماری بلاست در تعدادی از ارقام برنج. بیماری¬های گیاهی 46(1): 181-192.
مؤمني، ع.، پاداشت دهكايي، ف.، موسي نژاد، ص. و نيك¬خواه ج. م. 1385. تعیین اجزای مقاومت کاهنده بلاست در ارقام منتخب برنج. دانش کشاورزی 16(3): 135-144.
Cho, Y.C., Choi, I.S., Baek, M.K., Suh, J.P., Hong, H.C., Kim, Y.G., Koizumi, S., Jena, K.K., Choi, H.C. and Hwang, H.G. 2003. Resistant genes and their effects to rice blast in isogenic lines of genetic background of Chucheongbyeo and Suweon 345. Rice Genet. News 20: 101-105.
Cho, Y., Kwon, S., Choi, I., and Lee, S. 2007. Identification of major blast resistance genes in Korean rice varieties (Oryza sativa L.) using molecular markers. Journal of Crop Science10: 265–276
Jennings, P. R., L.E. Berrio, E. Torres and E. Corredor. 2003.A breeding strategy to increase rice yield potential. Webapp. Ciat. cigiar. org/biotechnology/pdf/Jennings/pdf Accessed on 2rd October.
Jeon, J.S., Chen, D., Yi, G.H., Wang, G.L. and Ronald, P.C. 2003. Genetic and physical mapping of Pi5(t), a locus associated with broad-spectrum resistance to rice blast. Molecular Genetic Genomics 269: 280-289.
Singh, A. K., Singh, P. K. Arya, M., Singh, N. K. and Singh. U. S. 2015. Molecular screening of blast resistance genes in rice using SSR markers. Plant Pathology Journal 31(1): 12–24.