Effect of Protein and Vitamins Supplements on Growth Indices of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae at blastospore proliferation stage

Subject Areas : entomology and othea arthropods

M.  latifian ¹ (رئیس پژوهشکده عضو هیأت علمی)
B.  rad ² (Date palm and tropical fruits research center)

Received: 2018-12-25 Accepted : 2019-06-24 Published : 2019-08-23

Keywords:

Abstract :

The success of any raw material used in the industrial and mass production of entomopathogenic fungi depends on the extent to which it can provide the optimum food requirement for the fungus. The biological control strategy using entomopathogenic fungi like B. bassiana and M. anisopliae can only be useful if practical and economic methods of mass multiplication are available. In this study, the effects of Protein and Vitamins Supplements on chlamydospores production of these two fungi were investigated. The results of this study showed that different treatments of Protein and Vitamins Supplements in complete liquid culture for chlamydespore cycle of B. bassiana and M. anisopliae fungi in terms of production spores, germination percentage, wet weight and dry weight difference Significant at 1% probability level. The highest concentration of chlamydosporum, germination percentage, wet weight and dry weight of B. bassiana and M. anisopliae were whey protein supplement and multivitamin at a concentration of 6 ml / liter. Also, the results of this study showed that these two species of pathogenic fungus can be replicated with good performance of sugarcane by products and maintain the germination capacity of chlamydospores.