Effect of salicylic acid treatment on common bean mosaic virus damage in bean variety Akhtar
Subject Areas : Plant PathologySasan Ghasemi 1 , Adeleh Abedi Firoozjaei 2
1 - دانشگاه آزاد اسلامی واحد شیراز
2 - Islamic Azad University, Shiaz Branch, Iran
Keywords: Disease severity, Elisa, Salicylic acid, BCMV, Bean,
Abstract :
Bean common mosaic virus and related races are one of the most important bean viral diseases and are present in almost all bean growing regions. In order to investigate the effects of salicylic acid treatment on the reaction of sensitive bean variety (Akhtar) to common bean mosaic virus (BCMV), an experiment was conducted in the form of a completely randomized design with 4 replications in greenhouse conditions. The experimental treatments included concentrations of zero, 200, 400 and 800 micromolar salicylic acid. The desired treatments were performed three days before inoculation with the respective virus agent. ELISA test using BCMV antiserum showed 83.3% of samples were infected with this virus. The results showed that different levels of salicylic acid could reduce the severity of the disease compared to the control infected with the virus.The lowest severity of the disease was obtained in the treatment of salicylic acid with a concentration of 800 micromolar (15.5%). The amount of phenol in the leaves in the treatment of salicylic acid with a concentration of 800 micromolar showed the highest value (92%), which did not show a statistically significant difference with the infected control, and this trait can be an indicator of the induction of resistance with the improvement of phenol levels due to the use of salicylic acid. Salicylic acid was effective in improving the morphological parameters such as the wet weight of the aerial parts of the plant and the dry weight of the root, and in disease conditions it prevented further damage during the experimental period to such an extent that this amount in the treatment of salicylic acid with a concentration of 800 µM in the conditions of the disease did not show any significant difference with the healthy control.
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