Antimicrobial and Healing Effects of Purslane, Green Tea and Mountain Tea Extracts on Burn Infection Caused by Staphylococcus aureus in Mice
Subject Areas : Experimental physiology and pathologyNader Kazemi 1 , Mona Ghasemi 2 , Mahdi Arfaei 3
1 - Nanobiotechnology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran
2 - Department of Biology, Faculty of Basic Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
3 - Department of Biology, Faculty of Basic Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
Keywords: Burn Infection, Camellia sinensis, Portulaca oleracea, Stachys lavandulifolia, ,
Abstract :
Introduction: Staphylococcus aureus are the cause of hospital infections and infectious diseases. The purpose of this research is to study the healing effect of ethanolic and acetonic extracts of Portulaca oleracea, Camellia sinensis and Stachys lavandulifolia with Eucerin on burn infection caused by Staphylococcus aureus in mice. Methods: After collecting and identifying the plants, the MIC and MBC levels of the ethanolic and acetonic extracts of the plants were determined by the dilution method in Mueller Hinton Broth culture medium. After causing 2nd degree burns in mice to infect the wound, inoculation of Staphylococcus aureus bacteria solution with a concentration of (5 × 105 CFU/ml) and then ointments prepared with MBC concentration from plant extracts per 1 gram of Eucerin, after 24 hours were used for treatment. Results: In the studies conducted on mice, it was found that Portulaca oleracea, Camellia sinensis and ethanol extracts and Stachys lavandulifolia extracts have stronger effects than green tea alcoholic extracts. Also, the alcoholic extract of purslane has the lowest effectiveness, and in the burn, site treated with the alcoholic extract of this plant, bleeding and a decrease in healing speed were observed. Conclusion: Ethanolic and acetonic extracts of Camellia sinensis, Stachys lavandulifolia and acetonic extract of Portulaca oleracea have antimicrobial and healing effects on burn infection. Among these, Portulaca oleracea acetonic extract, Camellia sinensis and Stachys lavandulifolia ethanolic extract have the most effect, which can be used as medicine or antimicrobial burn ointment.
