The effect of two types of exercise (continuous and intermittent) and atrostatin on the expression of PP2Ac and GSK-3β genes in the heart tissue of diabetic rats
Subject Areas :
Maryam Ebrahimi
1
(Department of Exercise Physiology, Ali Abad Katol Branch, Islamic Azad University, Ali Abad Katol,, Iran)
Habib Asgharpour
2
(Assistant Professor, Department of Exercise Physiology Aliabad Katol Branch, Islamic Azad University, Aliabad Katol.)
parvin farzanegi
3
(Associate Professor, Exercise Physiology Department, Sari Branch, Islamic Azad University, Sari, Iran)
Reza Rezaee Shirazi
4
(Assistant Professor, Department of Exercise Physiology Aliabad Katol Branch, Islamic Azad University, Aliabad Katol)
Keywords: atherostatin, Continuous and intermittent exercise, PP2Ac, GSK-3&beta, diabetic cardiomyopathy,
Abstract :
Background and Aim: Apart from high blood pressure and coronary artery disease, diabetes can directly affect the structure and function of the heart and lead to a complication called diabetic cardiomyopathy. The purpose of this study is to investigate the effect of two types of training and the drug atrostatin on the expression of PP2Ac and GSK-3β genes in the heart tissue of diabetic model rats.Methods and Materials: 64 male rats, which were randomly divided into 8 groups: including 1) control, 2) diabetic, 3) diabetic + persistent, 4) Diabetic + periodic, 5) atherostatin, 6) continuous + atrostatin, 7) periodic + atrostatin and 8) saline. The rats of the exercise groups performed two types of continuous and intermittent exercise 5 days a week for 8 weeks. Atrostatin was injected intraperitoneally daily at a dose of 20 mg per kg body weight.Results: Induction of diabetes led to a decrease in the expression of the PP2Ac gene and an increase in the expression of the GSK-3β gene in the heart tissue, and continuous exercise and the combination of intermittent and continuous exercise with the drug atrostatin led to an increase in PP2Ac and a decrease in GSK-3β in the heart tissue of diabetic rats compared to the diabetic group.Conclusion: It is possible that regular exercise in combination with atrostatin can prevent the development of diabetes-induced cardiomyopathy by increasing the PP2Ac gene and decreasing the GSK-3β gene in the heart tissue which has a protective effect on the heart of diabetic rats.
_||_