Molecular detection of coronavirus causing infectious bronchitis in laying hens with cystic oviduct and quantitative and qualitative reduction of egg production

Subject Areas :

Maryam Jalahi ¹ , Majid Gholami-Ahangarn ²

1 - Graduated of Veterinary Medicine Faculty, Shahrekord Branch, Islamic Azad University, Shahrekord, IRAN
2 - Department of Poultry Diseases, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Received: 2020-11-16 Accepted : 2021-04-19 Published : 2021-04-21

Keywords: Quantitative and qualitative reduction of egg production syndrome, Cystic oviduct, Isfahan, Infectious Bronchitis,

Abstract :

Background and Aim: In this study, the contribution of IB coronavirus in egg quantitative and qualitative reduction syndrome was investigated.Methods: nine laying hen flocks attected to egg quantitative and qualitative reduction syndrom and 10 laying farms with a healthy appearance were sampled. In addition, 6 farms with cystic oviduct were sampled in laying flocks, in Isfahan province. After extracting the genome, a fragment of 1200 bp of coronavirus S1 gene was amplified for identification of IB.Results: Out of 9 laying farms with quantitative and qualitative egg reduction syndrome and 10 apparently healthy farms, respectively 7 and 4 farms (78 and 40%) were infected with IB coronavirus based at least one positive sample. Out of 59 samples from farms with egg quantitative and qualitative reduction syndrome, 32 samples (54.2%) and out of 66 samples taken from apparently healthy farms, 7 samples (10.6%) were evaluated positive for IB coronavirus. In this study, all 6 farms with cystic oviduct had at least one positive sample for IB coronavirus and 31 samples out of 41 samples (73.17%) infected with IB coronavirus.Conclusion: IB coronavirus has a high share in the syndrome of quantitative and qualitative reduction of egg production in laying hens, but not all cases can be attributed to this virus and other infectious and non-infectious factors should be monitored. Due to the high frequency of this virus in cystic oviduct, it is necessary to identify the viral types that induce this disorder and to develop an appropriate control program.

References:

منابع

 

1-غلامی آهنگران، م.، چرخکار س.، و شوشتری ع.، بزرگمهری‌فرد م.ح، عشرت‌آبادی ف. 1387. شناسایی مولکولی و تعیین تیپ ویروس برونشیت عفونی در موارد سندرم تنفسی جوجه‌های گوشتی در استان اصفهان. مجله علوم دامپزشکی ایران. سال پنجم. شماره 3. ص 476-469.

2-غلامی آهنگران، م.، شوشتری ع.، دوستی ع.، فتحی‌هفشجانی ع.، ضیاء جهرمی، ن. 1391. شناسایی تیپ 91/4 ویروس یرونشیت عفونی در جوجه‌های گوشتی استان چهارمحال و بختیاری. مجله دامپزشکی دانشگاه آزاد اسلامی واحد تبریز. پیاپی 2. ص 1537-1533.

3.Aghakhan, S.M., Abshar, N., Rasoul Nejad Fereidouni, S., Marunesi, C., Khodashenas, M. (1994). Studies on avian viral infections in Iran, Arch Razi Inst, 46/47; 56-63.

4.Boroomand, Z., Jafari, R.A., Mayahi, M. (2018). Molecular detection and phylogenetic properties of isolated infectious bronchitis viruses from broilers in Ahvaz, southwest Iran, based on partial sequences of spike gene. Vet Res Forum, 9(3); 279-283.

5.Bozorgmehri-Fard, M.H., Charkhkar, S., Hosseini, H. (2013). Detection of the chinese genotype of infectious bronchitis virus (QX-type) in Iran. Iran J Virol, 7(1&2); 57-60.

6.Chousalkar, K.K., Roberts, A. (2009). Effects of Australian strains of infectious bronchitis virus on internal and external quality of hen eggs. Anim Prod Sci, 49; 162–169.

7.De Wit, J.J., Nieuwenhuisen-van Wilgen, J., Hoogkamer, A., van de Sande, H., Zuidam, G.J., Fabri, T.H.F. (2011). Induction of cystic oviducts and protection against early challenge with infectious bronchitis virus serotype D388 (genotype QX) by maternally derived antibodies and by early vaccination. Avian Pathol, 40(5); 463-471.

8.Ghalyanchi-Langeroudi, A., Karimi, V., Jannat, A., Hashemzadeh, M., Fallah, M.H., Gholami, F. (2015). Genotyping of infectious bronchitis viruses in the east of iran. Iran J Virol, 9(2); 1-5.

9.Ghalyanchilangeroudia, A., Hosseini, H., Fallah Mehrabadic, M.H., Ghafourid, S.A., Modiri Hamdana, A., Ziafatia, Z. (2019). Genotyping of avian infectious bronchitis virus in Iran: Detection of D274 and changing in the genotypes rate. Comp Immunol Microbiol Infect Dis, 65; 110–115.

10.Hughes, B.O., Gilbert, A.B., Brown, M.F. (1986). Categorisation and causes of abnormal egg shells: relationship with stress. Br Poult Sci, 27(2); 325–337.

11.Ignjatovic, J., Sapats, S. (2000). Avian infectious bronchitis. Rev Sci Tech off Int Epiz, 19(2); 493-508.

12.Jackwood, M.W. (2012). Review of infectious bronchitis virus around the world. Avian Dis, 56(4); 634-641.

13.Jackwood, M.W., de Wit, S. (2020). Infectious bronchitis. In: Swayne, D.E., Boulianne, M., Logue, C.M., McDougald, L., Nair, V., Suarez, D.L., editors. Diseases of Poultry. 14th ed. Wiley-Blackwell, Inc. p. 167–188.

14.Krapez, U., Slavec, B., Rojs, O.Z. (2011). Circulation of infectious bronchitis virus strains from Italy 02 and QX genotypes in Slovenia between 2007 and 2009. Avian Dis, 55(1); 155-161.

15.Miłek, J., Blicharz-Domańska, K. (2018). Coronaviruses in avian species – review with focus on epidemiology and diagnosis in wild birds. J Vet Res, 62; 249-255.

16.Sabato, L., Vaccari, G., Lelli, D., Lavazza, V., Castrucci, MR., Moreno A. (2019). A48 Identification and full-genome characterization of Alpha- and Beta-Coronaviruses viruses from bats in Italy. Virus Evolution, 5(10); 100-110.

17.Srinivasan, P., Balasubramaniam, G.A. (2011). Cystic dilatation of right oviduct in layer chicken. Tamil nadu J Vet Anim Sci, 7; 218-220.

18.Vasfi Marandi, M., Bozorgmehri Fard, M.H. (2000). Isolation and identification of infectious bronchitis virus in chicken in Iran. 21st World Poultry Congress, p. 100