Effect of β-sitosterol-enriched walnut oil on blood parameters and liver function in diabetic rats

Subject Areas :

Monireh  Ghorbani ¹ (DepatmentI of Biology, Damghan Branch, Islamic Azad University, Semnan, Iran.)
عبدالحسین  شیروی ² (Associate Professor, DepatmentI of Biology, Damghan Branch, Islamic Azad University, Semnan, Iran.)
Gholamhassan  Vaezi ³ (Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran)
Vida  Hojati ⁴ (Department of biology, Damghan branch, Islamic Azad Univercity, Damghan , Iran)
Hamid  Sepehri ⁵ (Golestan Neuroscience Research Center, Golestan Univercity of Medical Sciences, Gorgan, Iran.)

Received: 2020-05-17 Accepted : 2020-12-20 Published : 2020-12-21

Keywords: Walnut oil, liver, Diabetes, β-sitosterol,

Abstract :

Problems and complications associated with liver enzyme changes can be regarded as some effects of Diabetes Mellitus. Using a nutritional approach is a significant part of the prevention, control and treatment process. The aim of this study was to investigate the effect of walnut oil enriched with beta-cytosol on blood parameters of liver function in diabetic rats.The study sample consisted of 30 mice divided into 5 groups of 6 rats, where each group was kept in a separate cage. Five treatments were considered in this experiment, where control group received normal saline, the second treatment were given pure walnut oil without the addition of β-sitosterol and the other three groups were gavaged with 30, 45, 60 mg / kg β-sitosterol. After 4 weeks of gavage, the next stage of blood sampling was performed. Insulin and blood glucose were measured after blood serum separation along with the measurement of blood factors such as cholesterol, triglyceride, high-density cholesterol (HDL), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin, direct bilirubin, and albumin. Significant differences existed among the mean values of the tests in the control group and the intervention groups without, with 30 mg/kg, with 45 mg/kg, and with 60 mg/kg β-sitosterol. These significant differences among the treatments were seen in mean fasting blood sugar, cholesterol, triglyceride, high-density cholesterol (HDL), total aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin, direct bilirubin, and albumin.β-sitosterol-enriched walnut oil can be regarded as a helpful option to control diabetes.

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