Effect of green tea extract (Camellia sinensis) on levels of sex hormones in letrozole-induced polycystic ovary syndrome (PCOS) in adult female Wistar rats
Subject Areas :
ایمان
خدارحمی
1
(گروه زیست شناسی، دانشکده علوم پایه، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران)
اکرم
عیدی
2
(گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران)
پژمان
مرتضوی
3
(گروه پاتولوژی، دانشکده دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران)
Keywords: Polycystic ovarian syndrome, Camellia sinensis, Green tea, Rat, Letrozole,
Abstract :
Background and Objectives: Green tea (Camellia sinensis) was considered effective medicine for treating different ailments in ancient Asian folk medicine. It is known for its abundance of antioxidants. Polycystic ovary syndrome (PCOS) is a prevalent endocrinological disorder in reproductive-age women and is often associated with metabolic syndrome. The purpose of the study, the effect of green tea extract on letrozole-induced oxidative stress was investigated on levels of sex hormones of adult female Wistar rats. Materials and Methods: In this study, a total of 48 female rats were randomly divided into 8 groups of 6: The normal control group (intact), the healthy experimental group (green tea extract 50, 100 and 200 mg/kg bw, gavage), PCOS control group (letrozole 1 mg/kg bw, gavage), PCOS experimental group (green tea extract 50, 100 and 200 mg/kg bw together letrozole). The animals were euthanized 24 h after the last dose of the treatment on day 29. Blood was immediately obtained after the animals were sacrificed. The serum was used to assay the levels of testosterone, estradiol, FSH and LH. Results: Our results showed that administration of letrozole significantly increased the levels of testosterone, estradiol, LH and decreased FSH. The results study showed that green tea extract treatment significantly decreased the levels of testosterone, estradiol, LH and increased FSH in PCOS experimental group. Conclusion: These results provide data supporting the potential clinical use of green tea extract for the treatment of PCOS.
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