The effect of fennel extracted on the probiotic activities in the Drosophila melanogaster and study on the reproductive performance of male Drosophila melanogaster
Subject Areas :
ابوالحسن
رضایی
1
(گروه ژنتیک، دانشکده زیست شناسی، دانشگاه آزاد اسلامی واحد تنکابن، تنکابن، ایران)
مسعود
قانع
2
(گروه میکروبیولوژی، دانشکده زیست شناسی، دانشگاه آزاد اسالمی واحد تنکابن، تنکابن، ایران)
شیدا
اخشابی
3
(گروه ژنتیک، دانشکده زیست شناسی، دانشگاه آزاد اسالمی واحد تنکابن، تنکابن، ایران)
Keywords: Male Drosophila melanogaster, Probiotic, Fennel,
Abstract :
Inroduction & Objective: In the present study, the effect of fennel extract on Drosophila fertility and its role in probiotics were investigated.Probiotics with the activity of the gastrointestinal tract produce numerous vitamins and proteins that are needed for fertility in flies and in general in different organisms. Material and Method:Fennel extracts were divided into different concentrations. Concentrations containing 25, 50 and 75 mg / ml were added to the culture medium then the number of male spermatozoa were counted under light microscopy to be placed in A medium. Numbers of accessory gland cells, cell size and accessory gland size were observed by light microscopy. Also, accessory gland proteins were isolated and all data were analyzed by SPSS. PCR technique was also performed. Results: The mean number of sperm was 6440 and 3995, the number of accessory gland cells was 3502 and 1364, the size of accessory gland cells was 0.0675 and 0.0286 mm 2, the size of accessory gland was 0.339 and 0.391 cm 2, respectively. The protein of accessory gland 19/16 and 16/95 μg, respectively, were in the concentration of fennel (75 mg / ml) and concentration (25 mg / ml), respectively. The results of analysis showed that fennel had a significant effect on increasing fertility. It showed that a band of approximately 300 bp was observed on the gel. Conclusion: The results showed that when the higher concentration of fennel extract (75mg / ml) was used in the culture medium, the fertility rate in male Drosophila was higher than the lower concentration (25mg / ml) and in the control group. In the present study, the presence of probiotics indicates changes in the culture medium and its effect on fertility rate.
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