Gene expression pattern Of insulin-like growth factor–i receptor induced by tumor necrosis factor-αin human bone marrow derived mesenchymal stem cells
Subject Areas :Zainab Sahraiyan 1 , Maryam Ayatollahi 2 , Ramin Yaghobi 3
1 - دانشگاه آزاد اسلامی واحد علوم و تحقیقات فارس، دانشجوی دکتری زیست شناسی تکوینی، دانشکده علوم پایه، شیراز. ایران.
2 - استادیار دانشگاه علوم پزشکی شیراز، مرکز تحقیقات پیوند و ترمیم اعضاء بیمارستان نمازی، شیراز. ایران.
3 - دانشیار دانشگاه علوم پزشکی شیراز، مرکز تحقیقات پیوند و ترمیم اعضاء بیمارستان نمازی، شیراز. ایران.
Keywords: Human Bone Marrow, Insulin-Like Growth Factor-I Receptor (IGF-IR), Mesenchymal Stem Cells (MSCs), Tumor Necrosis Factor-Α (TNF-Α),
Abstract :
Inroduction and ObjectiveAcute liver failure (ALF) is a severe liver injury accompanied by sustained liver damage. It is imperative that new approaches for repairing the liver are developed. Cell-based therapy has been implicated in the treatment of liver diseases. Mesenchymal stemcells (MSCs) from various sources such as bone marrow are available. These cells are one of them ajorcandidates incell therapy.The production of insulin-like growth factor-I (IGF-I) increases in the regenerating liver. The IGF-I in liver regeneration is effective after binding to insulin-like growth factor-I receptor (IGF-IR).We hypothesized that tumor necrosis factor-αstimulate MSCs to cause IGF-IRexpression.Materials and Methods:Bone marrow was aspirated fromhuman normal donor after informing consent. Cells were isolated and cultured. Identification of cells with flow cytometric analysis was performed. Fourth passage cells were treated with tumor necrosis factor-α (TNF-α)at 1 ng/mL doseand incubated at different times (2, 10, 24 and 48 hours).The IGF-IRgene expression was investigatedusing real time-polymerase chain reaction technique.Results: Flow cytometric analysis showed that the human bone marrow MSCs were positive for CD90 and negative for CD45 and CD80.The IGF-IRgene expression was increased in TNF-α treated in comparison with untreated cells.Conclusion:Increase gene expression pattern of IGF-IR in human bone marrow derived MSCs may be used for clinical stem cell therapy in ALF.
