Study the Respiratory Burst Of Peripheral Blood Monocytes in the Presence Of Saccharomyces cerevisiae β–Glucan in Patients with Diabetes

Subject Areas :

Mohammad Hosain Arash  Asadirad ¹ (گروه میکروبیولوژی، واحد زنجان، دانشگاه آزاد اسلامی، زنجان. ایران)
Azadeh  Tofighi ² (گروه میکروبیولوژی، واحد زنجان، دانشگاه آزاد اسلامی، زنجان. ایران)
Ali  Shahnavaz ³ (گروه ریاضی و آمار، واحد زنجان، دانشگاه آزاد اسلامی، زنجان. ایران.)

Received: 2015-05-20 Accepted : 2018-08-04 Published : 2014-04-21

Keywords: Diabetes, Beta-Glucan, Saccharomyces cerevisiae, Oxidative Burst,

Abstract :

Inroduction and Objective: Diabetes mellitus is a chronic disease with epidemic potential. Diabetics are faced with complications of metabolic disorders and defects in their immune system. The use of immune system stimulating agents, such as beta-glucan is one of the researcher's interests to reduce the complications of diabetes. The present study aimed to investigate the effect of beta- glucan cell wall of Saccharomyces cerevisiae strains AT-3 on the respiratory burst of peripheral blood monocytes in patients with diabetes.Material and Methods: Peripheral blood of 53 patients with diabetes and 50 nondiabetic patients with an average of 39 years were sampled from Vali-Asr Hospital of Zanjan. Cells of Saccharomyces cerevisiae strain AT-3 were broken using sonikator. The cell wall was isolated. Beta- glucan was extracted from the wall. Nitro Blue Tetrazolium test was used to evaluate the respiratory burst of monocytes stimulated with beta glucan. Data were analyzed using multi-way ANOVA .Results:The results indicated that the respiratory burst of stimulated monocytes was increased in both men and women. The effect was dose dependent and it was significantly higher in women (pConclusion:Beta- glucan extracted fromSaccharomyces cerevisiae strain AT-3 isa potent immune system activator