Tissue and Molecule Investigation and the Effect of Copper Nanoparticles Synerigm with Antibiotics on Colorectal Cancer Tumors Infected to Kelebsiella pneumonia
Subject Areas :Sedighe Mehrabian 1 , Maria Valadbeaigi 2 , Shahla Mohammadganji 3
1 - استاد دانشگاه آزاد اسلامی واحد تهران شمال ودانشگاه خوارزمی، تهران. ایران.
2 - کارشناسی ارشد بیوتکنولوژی میکروبی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران. ایران.
3 - استادیار ژنتیک مولکولی پژوهشگاه ملّی مهندسی ژنتیک وزیست فنا وری، تهران. ایران.
Keywords: Colorectal cancer, Kelebsiella pneumonia, Synerigm, Copper nanoparticles, Anti-Biotic,
Abstract :
Inroduction & Objective: Colorectal is second common type of cancer in the universe. Genetic alteration scope in Colorectal is wide spread and inspections indicates infection to special kind of Kelebsiella pneumoniae bacteria which includes pks genes, can cause Colorectal cancer. A Kelebsiella bacterium is the reason for infectious diseases with challenging cures. Anti-biotic which are taken to cure (remedy) are of high side effects. This study is designed for the purpose of eliminating drug resistance and making use of low dose antibiotics in specific bacterial infections which makes change possible in intestine cell and incidence of cancer and may help cure or prevention.Material and Methods:In this method the investigation was carried out after supplying copper nanoparticles from Nano Pars Company with scale of 20 nanometer, purity degree of 99.9%, isolation and identification of Kelebsiella bacterium performed using common microbiologic and molecular methods. Kirby and Bauer method used in order to investigate bacterium resistance with different density of copper nanoparticles. The Synergistic effect or anti-biotic was investigated by impregnated anti-biotic discs with highest density of copper nanoparticles which not possess inhibitoringeffect. In this study strains including pks gene, were identified and isolated through PCR, then through different tests antimicrobial effects of copper nanoparticles and antibiotics upon mentioned strains was investigated. Antibiotics resistant to strains were identified. Mentioned bacteria sensitive to copper nanoparticles highest concentrations of copper nanoparticles that was not inhibited, concluded that using the lowest concentration of nanoparticles that demonstrated synergistic effects with antibiotic resistant.Results: The overall result can be used for treatment and prevention using nano biotic. In comparison with copper nanoparticles aforesaid bacteria were sensitive, highest density of copper nanoparticles which had no controlling effect, were selected.Conclusion:General result was that through using lowest density of nanoparticles which the impact of Synergistic with resistant anti-biotic indicated, nano-biotic can be used for curing and prevention.
