The effect of lycopene on testicular parameters in Mehraban ram

Subject Areas : zoology

Dehghani Ghobad ¹ , Saied Mohammadzadeh ² , Ali Kiani ³

1 - Master, Department of Animal Science, Faculty of Agriculture, Lorestan University, Lorestan, Iran
2 - Associate Professor, Department of Animal Science, Faculty of Agriculture, Lorestan University, Lorestan, Iran
3 - Associate Professor, Department of Animal Science, Faculty of Agriculture, Lorestan University, Lorestan, Iran.

Received: 2022-03-24 Accepted : 2022-07-28 Published : 2021-12-22

Keywords: Testis, Lycopene, Macroscopic parameter, ram, Microscopic parameter,

Abstract :

Objectives: Lycopene is an aliphatic hydrocarbon that has antioxidant properties and is capable to collect and neutralize free radicals. The purpose of this experiment was to investigate the effect of lycopene on the reproduction of Mehraban ram.Methods: In this experiment 20 male lambs aged 6 months, weighted 27±0.5 kg after clinical confirmation and vaccination (Food and mouth disease (FMD), Agalaxy and Enterotoxaemia) were selected. Lambs divided to 2 experimental groups with 10 replications. After two weeks of acclimatization, the lambs reared 100 days. The animal groups composed control (basic diet) and treatment basic diet + lycopene 5 gr/day. The lambs were fed twice per day, morning and evening. The water was freely available. At the end of the experimental period, after 24 hours of deprivation of feed, all lambs were slaughtered and testes were removed from the scrotum. Macroscopic traits were measured. Testis samples were fixed in formalin 10% for histology sections and microscopic features. Data of experiment were analyzed using a completely randomized design using SAS 2003 software and the means were compared with Duncan (P<0.05).Results: Application of lycopene significantly increased the thickness of the germinal epithelium and decreased the lumen diameter of seminiferous tubules compared to the control treatment (P˂0.05). No significant difference was observed in seminiferous tubules diameter and macroscopic traits.Conclusion: The results of this experiment showed that lycopene has positive effects on the seminiferous tubules parameters of testis in ram. 

References:

1. Moustafa MH, Sharma RK, Thornton J, Mascha E, Abdel‐Hafez MA, Thomas AJ & et al. Relationship between ROS production, apoptosis and DNA denaturation in spermatozoa from patients examined for infertility. Human Reproduction. 2004; 19(1): 129-38.
   DOI: https://doi.org/10.1093/humrep/deh024


2. Shi J, Maguer M. Lycopene in tomatoes: chemical and physical properties affected by food processing. Crit Rev Food Sci Nutr. 2000; 40(1): 1-42.
   DOI:1080/10408690091189275


3. Belgheisi S, Motamedzadegan A, Milani J, Rashidi L, Rafe A. Physicochemical characterization of different tomato commercial cultivars grown in Iran. Journal of food science and technology. 2020; 17(98): 73-84. DOI:29252/fsct.17.01.07


4. Hojjati M, Razavi SH. Review on lycopene characteristics and role of microorganisms on its production. IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY. 2011; 8 (29): 11-25.


5. Ellinger S, Ellinger J, Stehle P & Care M. Tomatoes, tomato products and lycopene in the prevention and treatment of prostate cancer: do we have the evidence from intervention studies? Current Opinion in Clinical Nutrition & Metabolic Care. 2006; 9(6): 722-7. DOI: 1097/01.mco.0000247470.64532.34.


6. Gürbüz M & Aktaç Ş. Understanding the role of vitamin A and its precursors in the immune system. Nutrition Clinique et Métabolisme. 2022; 36(2): 89-98.
   DOI:1016/j.nupar.2021.10.002


7. Kim HW, Chew BP, Wong TS, Park JS, Weng BBC, Byrne KM & et al. Dietary lutein stimulates immune response in the canine. Veterinary Immunology and Immunopathology. 2000; 74(3): 315-27. DOI: 1016/S0165-2427(00)00180-X


8. Ateşşahin, A & et al.Protective role of lycopene on cisplatin-induced changes in sperm characteristics, testicular damage and oxidative stress in rats. Reproductive Toxicology. 2006; 21(1): 42-47. DOI: 1016/j.reprotox.2005.05.003


9. Fallah R & Kiani A. The effect of lycopene and energy supplementation on serum and colostrum IgG concentrations in pregnant ewes. Journal of animal production. 2017; 19(3): 557-67. DOI: 22059/JAP.2018.223175.623146


10. Arballo J, Amengual J, Black M & Erdman J. Impact of Carotenoid Cleaving Enzymes on Lycopene Accumulation in Transgenic Mice. Current Developments in Nutrition. 2021; 5(Supplement_2): 69-69. DOI: 1093/cdn/nzab034_003.


11. Chiang H-S, Wu W-B, Fang J-Y, Chen D-F, Chen B-H, Huang C-C & et al. Lycopene inhibits PDGF-BB-induced signaling and migration in human dermal fibroblasts through interaction with PDGF-BB. Life Sci. 2007; 81(21-22): 1509-17.
    DOI:1016/j.lfs.2007.09.018.


12. Aly HA, El-Beshbishy HA & Banjar Z. Mitochondrial dysfunction induced impairment of spermatogenesis in LPS-treated rats: modulatory role of lycopene. Eur J Pharmacology. 2012; 677(1-3): 31-8. DOI: 1016/j.ejphar.2011.12.027


13. Moslemi E, Nouri M & Askari G. Effect of Lycopene Supplementation on Infertility in Men: A Systematic Review on Clinical Trial Studies. Muq- 2019; 12(12): 28-41. In Persian. DOI: 10.29252/qums.12.12.28


14. Morsi A, Shawky L & El Bana E. The potential gonadoprotective effects of grape seed extract against the histopathological alterations elicited in an animal model of cadmium-induced testicular toxicity. Folia Morphologica. 2020; 79(4): 767-76.
    DOI: 5603/FM.a2020.0003


15. Türk G, Ateşşahin A, Sönmez M, Yüce A & Ceribaşi AO. Lycopene protects against cyclosporine A-induced testicular toxicity in rats. Theriogenology. 2007; 67(4): 778-85. DOI: 1016/j.theriogenology.2006.10.013


16. Saemi F, Zamiri M, Akhlaghi A, Niakousari M, Dadpasand M & Ommati Dietary inclusion of dried tomato pomace improves the seminal characteristics in Iranian native roosters. Poultry Science. 2012; 91(9): 2310-5. DOI: 10.3382/ps.2012-02304


17. Fontoura A, Montanholi Y, de Amorim MD, Foster R, Chenier T & Miller S. Associations between feed efficiency, sexual maturity and fertility-related measures in young beef bulls. Animal: an international journal of animal bioscience. 2016; 10(1):
    96-105. DOI: 1017/S1751731115001925


18. Aly HJH & toxicology E. Testicular toxicity of gentamicin in adult rats: ameliorative effect of lycopene. Hum Exp Toxicol. 2019; 38(11): 1302-13.
    DOI: 1177/0960327119864160


19. Aly HA, Khafagy RMJF & Toxicology C. Taurine reverses endosulfan-induced oxidative stress and apoptosis in adult rat testis. Food Chem Toxicol. 2014; 64:1-9.
    DOI: 1016/j.fct.2013.11.007


20. Aitken RJ & Sawyer DJAimmdt. The human spermatozoon—not waving but drowning. Adv Exp Med Biol. 2003; 518: 85-98. DOI:1007/978-1-4419-9190-4_8


21. Aly H. Testicular toxicity of gentamicin in adult rats: ameliorative effect of lycopene. Hum Exp Toxicol. 2019; 38(11): 1302-13. DOI:1177/0960327119864160


22. Chen H, Pechenino AS, Liu J, Beattie MC, Brown TR & Zirkin BRJE. Effect of glutathione depletion on Leydig cell steroidogenesis in young and old brown Norway rats. Endocrinology. 2008; 149(5): 2612-9. DOI: 1210/en.2007-1245


23. Shrilatha BJRt. Early oxidative stress in testis and epididymal sperm in streptozotocin-induced diabetic mice: its progression and genotoxic consequences. Reprod Toxicol. 2007; 23(4): 578-87. DOI: 1016/j.reprotox.2007.02.001


24. Williams K, Frayne J, McLaughlin EA & Hall Expression of extracellular superoxide dismutase in the human male reproductive tract, detected using antisera raised against a recombinant protein. Mol Hum Reprod. 1998; 4(3): 235-42.
    DOI: 10.1093/molehr/4.3.235


25. Chandra J, Samali A & Orrenius S. Triggering and modulation of apoptosis by oxidative stress. Free Radic Biol Med. 2000; 29(3-4): 323-33.
    DOI:1016/s0891-5849(00)00302-6


26. Ling Y-H, Liebes L, Zou Y, Perez-Soler RJJoBC. Reactive oxygen species generation and mitochondrial dysfunction in the apoptotic response to Bortezomib, a novel proteasome inhibitor, in human H460 non-small cell lung cancer cells. J Biol Chem. 2003; 278(36): 33714-23. DOI: 1074/jbc.M302559200


27. He L, You S, Gong H, Zhang J, Wang L, Zhang C & et al. Cigarette smoke induces rat testicular injury via mitochondrial apoptotic pathway. Mol Reprod Dev. 2017; 84(10): 1053-65. DOI:1002/mrd.22863


28. Widmann C, Gibson S & Johnson GLJJoBC. Caspase-dependent cleavage of signaling proteins during apoptosis: a turn-off mechanism for anti-apoptotic signals. J Biol Chem.1998; 273(12): 7141-7. DOI: 1074/jbc.273.12.7141


29. Budihardjo I, Oliver H, Lutter M, Luo X & Wang XJAroc. Biochemical pathways of caspase activation during apoptosis. Annu Rev Cell Dev Biol.1999; 15(1): 269-90.
    DOI: 1081-0706/99/1115-0269


30. Burukoğlu D & Bayçu C. Protective effects of zinc on testes of cadmium-treated rats. Bull Environ Contam Toxicol. 2008; 81(6): 521-4. DOI: 1007/s00128-007-9211-x


31. Türk G, Ceribaşi AO, Sakin F, Sönmez M & Ateşşahin A. Antiperoxidative and anti-apoptotic effects of lycopene and ellagic acid on cyclophosphamide-induced testicular lipid peroxidation and apoptosis. Reproduction, fertility, and development. 2010; 22(4): 587-96. DOI:1071/RD09078


32. Qu M, Zhou Z, Chen C, Li M, Pei L, Chu F & et al. Lycopene protects against trimethyltin-induced neurotoxicity in primary cultured rat hippocampal neurons by inhibiting the mitochondrial apoptotic pathway. Neurochem Int. 2011; 59(8): 1095-103. DOI: 1016/j.neuint.2011.10.005


33. Khachik F, Carvalho L, Bernstein PS, Muir GJ, Zhao D-Y, Katz NBJEb & et al. Chemistry, distribution, and metabolism of tomato carotenoids and their impact on human health. Exp Biol Med (Maywood). 2002; 227(10): 845-51.
    DOI: 1177/153537020222701002