Evolution of bovine embryos cleavage in co-cultuer M.S.Cs deried from adipose tissues rat
Subject Areas : zoologyMehdi Ahmadifar 1 , علی محمدعینی 2 , Reihane Nateghi 3 , Nazila وحیدی ایریسفلی 4
1 - Royan Institute
2 - Young Researchers and Elite Club, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - دانشجوی دکتری فیزیولوژی دام، دانشکده علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران
4 - دانشکده زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، سمنان، ایران
Keywords: Mouse, co-cultured, Stem cell,
Abstract :
ObjectiveMesenchyme Stem cells (MSCs) are one of the widely used sources in tissue engineering and efficacy of cultural environment. In this survey, rat stem cells are used as coculture. Owing to main obstacle of embryo culture in the lab medium, the survival of embryo in lab is low in comparison with that of normal conditions. The purpose of this research is the survey of fertilization and culture of bovine embryos in mesenchymal stem cells derived from adipose tissue (ADMCs).ApproachIsolated ADMCs were cultured on 4-well dishes. Three days after cell culture, the bovine immature oocytes were transferred on this mono layer system and the in vitro matured oocytes were fertilized by sperms in another MSCs free environment subsequently. Then in vitro fertilized embryos were co-cultured with the same mono layer of ADMCs for seven days. During oocyte and embryo co-culture, the rates of maturation and cleavage were assessed.AchievementsThis study reveals that co-culture of embryos with mesenchymal stem cells could increase the survival and cleavage of embryos significantly (P<0.05). The maturation rate of bovine co-cultured oocytes (%82) in comparison with that of control group (%78) has increased significantly. Also, the rate of blastocyst formation in co-cultured embryos (%33) was analytically comparable with that of control group (%26).ConclusionWhat bolsters substantially the quality and development of embryo is coculture systems.
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