Effect of Culic® Herbal Medicine on Experimental Oral Salmonella Typhimurium Infection in Broilers
Subject Areas :
مائده تاجی
1
,
حمید استاجی
2
,
سحر غفاری خلیق
3
,
سید حسام الدین عمادی چاشمی
4
1 - دانش آموخته کارشناسی ارشد گروه زیست فناوری میکروبی، دانشکده بیوفناوری، دانشگاه سمنان، سمنان، ایران
2 - گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه سمنان
3 - گروه پاتوبیولوژی، دانشکده دامپزشکی دانشگاه سمنان، سمنان، ایران
4 - علوم بالینی/دانشکده دامپزشکی/ دانشگاه سمنان/سمنان/ایران
Keywords: Antibiotic Erythromycin, Salmonella Typhimurium, Culic® Syrup, Herbal Medicines, qPCR,
Abstract :
Salmonella typhimurium is one of the common serotypes of Salmonella, which is often associated with public health and economic losses worldwide. Combination therapy is a strategy to deal with antimicrobial resistance. The anti-cough syrup of Culic® commercialization includes effective ingredients of Thymus vulgaris, Glycyrrhiza glabra and Pimpinella anisum. Culic® syrup has antimicrobial and antiviral properties and is effective in treating digestive problems. The aim of this study is to investigate the antibacterial effect of Culic® syrup and the antibiotic erythromycin, as well as the combined effect of these two substances in the treatment and control of S. typhimurium infection in broilers. In this study, 40 one-day-old chicks of Ross 308 were infected with the standard strain of S. typhimurium. After oral inoculation of bacteria, chickens were randomly divided into equal groups: 1- no treatment, 2- treatment with erythromycin antibiotic, 3- treatment with Culic® and 4- erythromycin and Culic®. 24 hours after the induction of infection, 5 chickens from each group were subjected to microbiological examinations through colony counting and quantitative Real-Time PCR test and histopathological examinations after hematoxylin-eosin staining. The results of colony counting and Realtime-PCR in the studied groups showed that the control group had the highest bacterial load. After that, the highest bacterial load was assigned to the Culic® group and the erythromycin group, and the difference between these two groups was not significant. Finally, the lowest bacterial load was assigned to the treatment group with Culic® and erythromycin. The results of histopathology showed that the control group had the most tissue damage and a significant difference was observed between the control group and other groups. Other studied groups showed similar results, except the complication of liver necrosis in the erythromycin and Culic® group on the twelfth day, which had a significant difference with the other three groups. Therfore, Culic® herbal medicine has the same function as erythromycin and is effective in reducing the population of S. typhimurium bacteria synergistically with erythromycin
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