Isolation and identification of Mycobacterium tuberculosis from mice in a farm with tuberculosis reactor in Tehran province
Subject Areas :خشایار منصوری 1 , نادر مصوری 2 , مهدی بابائی 3 , شجاعت دشتی پور 4
1 - دانشکده دامپزشکی، واحد گرمسار، دانشگاه آزاد اسلامی، گرمسار، سمنان، ایران
2 - آزمایشگاه رفرانس سل گاوی، موسسه تحقیقات واکسن و سرم‌سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی (تات)، کرج، ایران
3 - آزمایشگاه رفرانس سل گاوی، موسسه تحقیقات واکسن و سرم‌سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی (تات)، کرج، ایران
4 - آزمایشگاه رفرانس سل گاوی، موسسه تحقیقات واکسن و سرم‌سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی (تات)، کرج، ایران
Keywords: Tuberculosis, Mice, Mycobacterium, RFLP-PCR, RD Typing,
Abstract :
Mycobacterium tuberculosis complex, the agent of tuberculosis, causes morbidity and mortality particularly in developing countries. It is important to investigate the possibility of mycobacterium transmission to animals such as mice, because as a result of the bacterial transmission to mice, the population of infected mice increases in animal husbandry and makes it possible to eliminate the entire livestock population.
DNA-fingerprinting tools have been developed to improve tuberculosis case detection and control. Molecular typing techniques allow to detect and follow M. tuberculosis complex. The aim of this study was to isolate and identify of Mycobacterium from mice with tuberculosis reactors. Five mice are selected from a Mycobacterium bovis-infected farm in 2019. To investigate the presence of Mycobacterium, the samples are cultured in Löwenstein-Jensen medium. PCR technique based on 16S rRNA and IS6110 fragment is used to identity of the isolates. Finally, Mycobacterium species are identified using RD-typing. After examining the resulting slide from the cultured samples, it is found that five mice are infected with Mycobacterium. Results of 16S rRNA and IS6110 PCR analysis shows the presence of 543 and 245 bp bands respectively, which supports that the isolates belonged to the Mycobacterium tuberculosis complex. The RD-typing technique confirms the presence of Mycobacterium tuberculosis in isolates. Animals such as mice can transmit various species of Mycobacterium tuberculosis complex to other farm animals and living organisms.
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