Observation of anti-cancer effects of aflatoxin B1 in breast tumor category 4T1 of mice by injection around the tumor

Subject Areas : Journal of Comparative Pathobiology

R. Mousazadeh, ¹ , S. Hesaraki, ² , M. Bayat, ³ , A. Jahandideh, ⁴ , J. Hashemi, ⁵

1 - Graduated from Faculty of Veterinary Medicine, Sciences and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - Department of Clinical Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran
5 - Department of Parasitology and Mycology, School of Health University of Medical Sciences University of Tehran, Tehran, Iran

Received: 2022-05-21 Accepted : 2022-05-21 Published : 2021-11-22

Keywords:

Abstract :

Treatment of breast cancer has many problems due to the systemic effects of chemotherapy. The use of certain molecules with cell killing effects, such as aflatoxin B1, can have fatal effects on breast cancer cells. In this study, we investigated the inhibitory effect of topical aflatoxin B1 use on breast cancer. Class 4T1 cancer cells were inoculated subcutaneously in female mice. Doses of 50 and 500 μg / ml of aflatoxin B1 were then injected around the tumors in comparison with doxorubicin. The ratio of tumor size to animal weight in different groups was evaluated along with histopathology by immunofluorescence on Ki-67 and VEGF indices. Aflatoxin B1 significantly reduces tumor volume, necrosis and suppresses angiogenesis in the tumor. However, liver metastasis occurred in all groups. Aflatoxin B1 in topical injections of 50 and 500 μg / ml around the tumor has anti-cancer effects on the breast. But it can not prevent metastasis.Aflatoxin-induced hepatotoxicity was also observed.

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