Optimization of the micropropagation protocol for the UCB1 pistachio rootstock
Subject Areas :
1 -
Keywords: shoot regeneration, optimization, Micropropagation, pistachio rootstock. ,
Abstract :
Rootstock propagation is one of the most important applications of tissue culture and this industry has been expanding in recent years. UCB1 root stock pistachio is also one of the important rootstocks that is economically important for agriculture due to its good attributesits. Considering its signifigence in agriculture industry, a study that determines the optimization of the micropropagation process of UCB1 pistachio in tissue culture conditions should be considered. The present study was conducted to develop an efficient protocol for in vitro organogenesis of pistachio through various optimizations of growth regulators. MS medium containing BAP (2mg/l) was the most suitable combination for bud establishment. shoots were cut into approximately 5-centimeter pieces and used in MS culture media containing 2 mg/l BAP Then, they were transferred to culture medium containing different concentrations of BAP (1-0.5-1.5-2 mg/L). The highest growth and regeneration rate of branches were observed in the DKW medium containing 1.5 mg/l BAP. The shoots were rooting in the medium with 2 mg/l IBA after the second cultivation and compatibility was done with 80% survival in peat moss and perlite. The results of the studies also showed that best regeneration in the culture medium containing 1.5 mg/l BAP was observed
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