Optimization of chlorophyll bioactive compound and polysaccharide of Enteromorpha intestinalis algae in the Caspian Sea

Subject Areas : Microbial Biotechnology

Somayeh Rahaiee ¹ , Rashid Alijani Ardashir ² , Fatemeh Razaghi ³

1 -
2 - Department of Microbial Biotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran.
3 - Department of Microbial Biotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran.

Received: 2025-03-01 Accepted : 2025-05-24 Published : 2025-06-10

Keywords: Algae, Polysaccharides, Central composite design, Chlorophyll, Solvents.,

Abstract :

Background & Objectives: Green algae pigments and polysaccharides have economic importance and therapeutic applications. Considering the abundance of green algae Enteromorpha intestinalis on the shores of the Caspian Sea, this study tried to investigate the efficiency of polysaccharide extraction and the optimal conditions of chlorophyll extraction.

Materials and methods: After sampling the algae from the seashores in the autumn season and washing it, the samples were dried in an oven, and then ethanol was used as a solvent to extract chlorophyll. The surface-response method (RSM-CCD, Design Expert v 12 software) (factors including the solvent concentration (70-96%), extraction time (60-120 min), and temperature (30-70 ℃) was used to determine the optimal conditions for chlorophyll extraction. The sample was first dried and suspended in ethanol to extract polysaccharides, and then solvents, including water, sodium hydroxide, and hydrochloric acid solvents were used.

Results: The surface-response method's results showed that the optimal conditions for maximum chlorop,hyll extraction were a solvent concentration of 96%, an extraction temperature of 61°C, and an extraction time of 84 min. After 24 h, the highest rate of polysaccharide extraction (82%) from green algae was obtained using a hydrochloric acid solvent.

Conclusion: This study's results generally showed that Enteromorpha green algae have a good potential for extracting chlorophyll pigment and polysaccharides.

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