Effect of Different Levels of Guanidinoacetic Acid and Lysine Supplementation on Performance, Blood Parameters, Carcass Characteristics and Myogenin Gene Expression of Arian Broilers

Subject Areas :

B. Aghayari Far ¹ , A.A. Saki ² , M.H. Nemati ³ , A. Hosseini ⁴

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2 - Department of Animal Science, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Iran
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4 -

Received: 2025-01-04 Accepted : 2025-06-11 Published : 2025-06-30

Keywords: boilers, blood metabolites, guanodinoacetic acid, L-lysine,

Abstract :

This study investigates the effects of guanidinoacetic acid (GAA) and high levels of lysine supplementation on performance, blood parameters, carcass characteristics, and myogenin gene expression (MYOG) in broiler chickens. A total of six hundred Arian day-old broiler chickens were arranged in a completely ran-domized design with a factorial arrangement, comprising 8 treatments, 5 replicates, and 15 chicks in each. Treatments included four levels of GAA (0, 0.3, 0.6, and 1.2 g/kg diet) and two levels of L-lysine (the Na-tional Research Council (NRC) recommended level and 20% higher than the NRC recommendation [Lys20]). The results showed that a significant effect on increasing body weight and improving the feed conversion ratio (FCR) by 0.3 g/kg GAA in diet (P<0.05). A significantly reduced feed intake and im-proved FCR by 20% increase lysine levels in the diet. The significant effect on body weight gain, reduced feed intake, and improved FCR (P<0.05) were observed by 0.3 g/kg GAA along with Lys20. In addition, significantly increased in breast muscle percentage (P<0.05). Blood parameters indicated that increased high-density lipoprotein (HDL) and decreased Low-density Lipoprotein (LDL) cholesterol levels by adding 1.2 g/kg GAA in diet, while increased HDL levels by Lys20 in this respect. The results showed that the highest level of MYOG gene expression was observed by interaction effect of 0.3 g/kg GAA and Lys20. Overall, the results indicated that improved the performance of broilers was pointed out by adding 0.3 g/kg diet of GAA along with Lys20.

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