Cloning and phylogenetic analysis of Bovine immunodeficiency virus gag gene in Iranian isolates
Subject Areas : microbiology
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Keywords: Bovine immunodeficiency virus, gag gene, pTZ57R/T vector, pGEX-4T-3 vector, Phylogenetic analysis,
Abstract :
Bovine immunodeficiency virus (BIV), a member of the family Retroviridae, genus Lentivirus, has three important genes namely gag, pol and env. In the first step, PCR product of gag gene of BIV, isolated in different regions of Iran, were cloned in to a pTZ57R/T vector; then insert were digested by BamHI and XhoI restriction enzymes and then cloned in to pGEX-4T-3 as an expression vector. Analysis of the partial bovine immunodeficiency virus (BIV) gag gene sequences obtained from insert and was run. The Iranian BIV gag sequence was compared to 5 other corresponding sequences of BIV isolated in different countries. In the end, It was revealed that nucleotide analysis of the sequences witnessed, a variation of 0.4-10.6% and constructing phylogenetic tree revealed two clusters in it.
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