Comparison of the Therapeutic Effects of Nano-Essence of Boswellia with the Cream of Terbinafine %1 in Guinea Pig Experimentally Infected by Microsporum Canis
Subject Areas : Smal Animal Medicine
morteza Razaghi manesh
1
,
Bahador Bardshiri
2
,
Shadi luine Nasab
3
,
Ayda Sharifiyan
4
,
شقایق قناد
5
1 -
2 -
3 -
4 -
5 -
Keywords: Nano Essence, Frankincense, Terbinafine, Dermatophytosis, Microsporom Kenis,
Abstract :
Nowadays, due to their antimicrobial and antioxidant properties and lower side effects, plant essential oils have gained widespread application. Nanotechnology, by converting these compounds into nanoparticles, imparts new characteristics to them. This study evaluated the efficacy of a frankincense nanoemulsion and 1% terbinafine cream in treating experimental dermatophytosis caused by Microsporum canis in guinea pigs through in vitro and in vivo methods.
The Minimum Inhibitory Concentration (MIC) was determined using the Broth Microdilution method following the CLSI M38-A protocol, and the MIC of the frankincense nanoemulsion was found to be 210 µg/mL. Eighteen guinea pigs were randomly divided into three groups of six, and treatment began five days post-infection, administered every 12 hours for 40 days. Data analysis was performed using the Kruskal-Wallis test and SPSS software.
Statistical analysis revealed significant differences between the terbinafine and negative control groups throughout the treatment period. Initially, the infection level in the nanoemulsion group was higher than in the terbinafine group. Comparison between the nanoemulsion and negative control groups demonstrated significant differences on all days except day 40 (p<0.05). Additionally, significant differences between the nanoemulsion and terbinafine groups were observed on days 19, 26, 33, and 40 post-infection (p<0.05).
These results confirm the antifungal efficacy of frankincense nanoemulsion against Microsporum canis and suggest its superiority over topical terbinafine treatment.
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