Effects of the histone deacetylase inhibitor Trichostatin A on morphology, conidiation and vegetative growth of Fusarium graminearum, the causal agent of wheat Fusarium head blight

Subject Areas : Plant Pathology

Shiva Amin ¹ , Saeed Rezaee ² , Amir Mousavi ³ , Hamid Reza Zamanizadeh ⁴

1 - Department of Plant Protection, Faculty of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Plant Protection, College of Agriculture and Food Industries, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 - Department of Plant Molecular Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
4 - Department of Plant Protection, Faculty of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University, Tehran, Iran

Received: 2024-06-23 Accepted : 2024-09-11 Published : 2025-01-07

Keywords: Chromatin, Deacetylation, Epigenetics, Triticum aestivum, TSA,

Abstract :

Acetylation is an important mechanism to regulate gene expression at a post-translational level. The process is catalyzed by histone acetyltransferases (HATs) and histone deacetylases (HDACs). HDACs play an essential role in fungal pathogenesis, response to environmental stress, and production of secondary metabolites. Therefore, this study aims to investigate the possible effects of Trichostatin A (TSA), a histone deacetylase inhibitor, on the morphology of Fusarium graminearum, the causal agent of Fusarium Head Blight disease. The fungus was grown on PDA medium supplemented with TSA at different dosages of 1.5, 3, and 10 µg mL-1. The vegetative growth and morphological characteristics of the fungi were evaluated on days 3, 5, and 7 after inoculation. Radial growth of the fungi was significantly suppressed by TSA after three days of exposure. Compared to the control, the lowest mycelial growth was observed in culture media supplemented with 10 µg mL-1 TSA on day seven. Additionally, TSA caused aerial hyphae formation. Conidia production decreased in response to all concentrations of TSA, and the minimum conidiation occurred in the highest concentration (10 µg mL-1). The results showed the role of epigenetic modifications in F. graminearum gene expression and may help to provide new strategies to manage Fusarium head blight disease.

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