Genotyping of isolated Salmonella enteritidis separated from foodstuffs by RAPD-PCR
Subject Areas : microbiologyfatemeh parsa 1 , Manouchehr momeni shahraki 2 , fatemeh Khodaverdipour 3
1 - Department of Biology, ShK.C., Islamic Azad University, ShahreKord, Iran
2 - Research Center of Nutrition and Organic Products (R.C.N.O.P), ShK.C., Islamic Azad University, Shahrekord, Iran
3 - Research Center of Nutrition and Organic Products (R.C.N.O.P), ShK.C., Islamic Azad University, Shahrekord, Iran.
Keywords: Salmonella enteritidis, Genotyping, RAPD-PCR.,
Abstract :
Salmonellosis is a common disease in humans and animals. One of the most important pathogens in humans, livestock and poultry is Salmonella enteritidis, a common cause of foodborne diseases. Conventional culture, biochemical and serological methods have shown little value for molecular analysis and subtraction, but molecular typing techniques such as RAPD-PCR provide different patterns for differentiating Salmonella species. The purpose of this study was to differentiate and distinguish between isolates of Salmonella enteritidis by RAPD-PCR. In 2018, 350 samples of red meat and chicken, vegetable, salad dressing and salad dressing were collected from Shahrekord's food and grocery centers. In the shortest possible time and in sterile conditions, the microbiology laboratory of Shahrekord Islamic Azad University It was transmitted. PCR isolation and confirmation were used to genotype isolates of Salmonella enteritidis using RDPD-PCR primer P1254 CCGCAGCCAA primer. 28 isolates of Salmonella enteritidis were isolated. At the level of similarity, 52% of the isolates studied had 17 subtypes with a similarity level of 76%. The number of subgroups was 24 subgroups, and the number of subgroups increased 95% to 15 subgroups, indicating a high genetic diversity among the isolates studied. In this way, 30 isolates are studied in 24 profiles
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