Effect of Artificial Deterioration on Germination Characteristics and Use of Seed Reserves in Bean
Subject Areas : Plant physiology
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Keywords: Seed deterioration, seed reserves, seed quality, beans,
Abstract :
This study was conducted to investigate the effect of artificial deterioration on seed germination and heterotrophic growth of bean seedlings. The experiment was conducted in a completely randomized design with four replications. The treatments included different levels of artificial deterioration for 12, 24, 36, 48, 60, 72 and 84 hours. The results showed that the effect of accelerated deterioration treatment on the traits of seed germination percentage, seed germination rate, electrical conductivity, rootlet length, shoot length, seedling dry weight, seed vigor index, remaining cotyledon dry weight, reserve utilization rate, seed reserve utilization efficiency and fraction of mobilized reserves was significant. The results of mean comparisons showed that increasing the duration of seed deterioration led to a decrease in the percentage and rate of seed germination, so that in the 84-hour deterioration condition, the percentage of germination decreased from 96 to 21 percent and the rate of germination decreased from 0.033 to 0.018 per hour. The electrical conductivity also increased with increasing the duration of natural storage due to the inability of the membranes to maintain their integrity and reached 71 µs/cm in the 84-hour deterioration condition. With increasing the severity of deterioration from 12 to 84 hours, the length of the radicle and stem decreased and it was observed that the length of the radicle and stem in these two treatments was 2.5 and 2.8 cm, respectively. With increasing the duration of seed deterioration from 12 to 84 hours, the weight of the remaining cotyledons of bean seeds increased so that the highest cotyledon weight of 186 mg was obtained in the artificial deterioration treatment of 84 hours..
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