A Comparative Study of Quantitative and Qualitative Sperm’s Parameters and Testicular Histomorphometric Features in Arian and Ross 308 Breeding Roosters
Subject Areas : Veterinary Clinical Pathologyءشدخخزاثاق َممغئثاق 1 , Loghman Ahmadzadeh 2 , Mohammad Hossein Alavi 3 , علیرضا طالبی 4 , مزدک رازی 5
1 -
2 - Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
3 - Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
4 - بیماری های طیور دانشگاه ارومیه
5 - گروه بافت شناسی مقایسه ای، دانشکده دامپزشکی، دانشگاه ارومیه
Keywords: Arian, Broiler breeder males, Ross308, Testis.,
Abstract :
Reduced fertility with advancing age is one of the main challenges in broiler breeder production. Since no comparative study has evaluated the fertility characteristics of the Arian strain alongside Ross 308, This study investigated sperm indices (motility, viability, and count) and testicular histomorphometry at 35, 45, and 55 weeks of age in both strains. Sperm and testicular samples were collected from 60 roosters (30 Arian, 30 Ross 308). The results of this study indicated that at 35 weeks, Arian had a higher (not significant) sperm count than Ross 308; at 45 and 55 weeks, no significant difference was observed, and at 55 weeks, Arian again exhibited a non-significant increase. In both strains, sperm count declined with age. At 35 weeks, sperm viability was higher in Ross 308, but at 45 weeks there was no significant difference; whereas at 55 weeks, Arian showed relatively higher viability. Sperm motility was higher in Ross 308 at 35 weeks but was greater in Arian at 45 and 55 weeks. Histological assessments revealed no significant differences between the strains regarding spermiogenesis, tubular differentiation, Sertoli cell counts, meiotic cells, or seminiferous tubule diameter. Sperm parameters in both strains showed a declining trend with age. Overall, Arian’s fertility was comparable to that of Ross 308 and demonstrated superior performance at older ages.
• Alavi, M.H., Allymehr, M., Talebi, A. and Najafi, G. (2020). Comparative effects of nano-selenium and sodium selenite supplementations on fertility in aged broiler breeder males. Veterinary Research Forum, 11(2): 135-141. doi: 10.30466/vrf.2018.83172.2093
• Anonymous. (2022). Arian Chicken. https://kimiakav.com/arian.
• Anonymous. (2020). Nutrition and Feed Management for Ross 308 AP Parent Stock in North America. https://en.aviagen.com/assets/Tech_Center/Ross_Tech_Articles/RossNote-Ross308AP-FeedandNutrition-2020-EN.pdf.
• Avital-Cohen, N., Heiblum, R., Argov-Argaman, N., Rosenstrauch, A., Chaiseha, Y., Mobarkey, N., et al. )2013(. Age-related changes in gonadal and serotonergic axes of broiler breeder roosters. Domestic Animal Endocrinology, 44(3): 145-150.
• Cerolini, S., Kelso, K.A., Noble, R.C., Speake, B.K., Pizzi, F. and Cavalchini, L.G.) 1997(. Relationship between spermatozoan lipid composition and fertility during aging of chickens. Biology of Reproduction, 57(5): 976-980.
• Choo, Y.K., Oh, S.T., Lee, K.W., Kang, C.W., Kim, H.W., Kim, C.J., et al. )2014(. The Growth Performance, Carcass Characteristics, and Meat Quality of Egg-Type Male Growing Chicken and White-Mini Broiler in Comparison with Commercial Broiler (Ross 308). Korean Journal for Food Science of Animal Resources, 34(5): 622-629.
• Collett, S.R. and Smith J.A. (2020). Principles of disease prevention, diagnosis, and control. In: Diseases of Poultry. Swayne, D.E. Edithor-in-Chief 14th ed., NJ: Wiley Blackwell, PP: 29-33.
• De Reviers, M., Williams, J., Garreau, M. and Brillard, J. )1981(. Predicting the adult daily sperm output after the first ejaculates in cockerels raised under different photoschedules. Reproduction Nutrition Développement, 21(6B): 1113-1124.
• Douard, V., Hermier, D., Magistrini, M. and Blesbois, E. )2003(. Reproductive period affects lipid composition and quality of fresh and stored spermatozoa in turkeys. Theriogenology, 59(3-4): 753-764.
• Franzo, G., Legnardi, M., Faustini, G., Tucciarone, C.M. and Cecchinato, M. )2023(. When everything becomes bigger: big data for big poultry production. Animals (Basel), 13(11): 1804.
• Hocking, P. )1989(. Effect of dietary crude protein concentration on semen yield and quality in male broiler breeder fowls. British Poultry Science, 30(4): 935-945.
• Hocking, P.M. and Bernard, R. )1997(. Effects of dietary crude protein content and food intake on the production of semen in two lines of broiler breeder males. British Poultry Science, 38(2): 199-202.
• Hocking, P.M. and Bernard, R. )2000(. Effects of the age of male and female broiler breeders on sexual behaviour, fertility and hatchability of eggs. British Poultry Science, 41(3): 370-376.
• Kang, J.K., Lee, Y.J., No, K.O., Jung, E.Y., Sung, J.H., Kim, Y.B., et al. )2002(. Ginseng intestinal metabolite-I (GIM-I) reduces doxorubicin toxicity in the mouse testis. Reproductive Toxicology, 16(3): 291-298.
• Karaca, A., Parker, H. and McDaniel, C. )2002(. Elevated body temperature directly contributes to heat stress infertility of broiler breeder males. Poultry Science, 81(12): 1892-1897.
• Parker, H.M., Yeatman, J.B., Schultz, C.D., Zumwalt, C.D. and McDaniel, C.D. )2000(. Use of a sperm analyzer for evaluating broiler breeder males. 2. Selection of young broiler breeder roosters for the sperm quality index increases fertile egg production. Poultry Science, 79(5): 771-777.
• Rezvanfar, M.A., Sadrkhanlou, R.A., Ahmadi, A., Shojaei-Sadee, H., Rezvanfar, M.A., Mohammadirad, A., et al. )2008(. Protection of cyclophosphamide-induced toxicity in reproductive tract histology, sperm characteristics, and DNA damage by an herbal source; evidence for role of free-radical toxic stress. Human and Experimental Toxicology, 27(12): 901-910.
• Romero-Sanchez, H., Plumstead, P., Leksrisompong, N., Brannan, K. and Brake, J. )2008(. Feeding broiler breeder males. 4. Deficient feed allocation reduces fertility and broiler progeny body weight. Poultry Science, 87(4): 805-811.
• Rosenstrauch, A., Allan Degen, A. and Friedländer, M. )1994(. Spermatozoa retention by Sertoli cells during the decline in fertility in aging roosters. Biology of Reproduction, 50(1): 129-136.
• Rosenstrauch, A., Weil, S., Degen, A.A. and Friedländer, M. )1998(. Leydig cell functional structure and plasma androgen level during the decline in fertility in aging roosters. General and Comparative Endocrinology, 109(2): 251-258. doi: 10.1006/gcen.1997.7029
• Sarabia Fragoso, J., Pizarro Díaz, M., Abad Moreno, J.C., Casanovas Infesta, P., Rodriguez‐Bertos, A. and Barger, K. (2013). Relationships between fertility and some parameters in male broiler breeders (body and testicular weight, histology and immunohistochemistry of testes, spermatogenesis and hormonal levels). Reproduction in Domestic Animals, 48(2): 345-352.
• Sexton, K., Renden, J., Marple, D. and Kempainen, R. )1989(. Effects of dietary energy on semen production, fertility, plasma testosterone, and carcass composition of broiler-breeder males in cages. Poultry Science, 68(12): 1688-1694.
• Shanmugam, M., Vinoth, A., Rajaravindra, K. and Rajkumar, U. )2014(. Evaluation of semen quality in roosters of different age during hot climatic condition. Animal Reproduction Science, 145(1-2): 81-85.
• Tabatabaei, S., Chaji, M. and Mohammadabadi, T. )2010(. Correlation between age of rooster and semen quality in Iranian indigenous broiler breeder chickens. Journal of Animal and Veterinary Advances, 9(1): 195-198.
• Talebi, A., Alimehr, M., Alavi, M.H., Najafi, G. and Simaei, N. )2018(. Comparative study of semen traits and histomorphometric features of testes of broiler breeder males with different phenotypic traits. Veterinary Research Forum, 9(1):1-6.
• Weil, S., Rozenboim, I., Degen, A.A., Dawson, A., Friedländer, M. and Rosenstrauch, A. )1999(. Fertility decline in aging roosters is related to increased testicular and plasma levels of estradiol. General and Comparative Endocrinology, 115(1): 23-28.
• Wilson, F.D., Johnson, D.I., Magee, D.L. and Hoerr, F.J. )2018(. Testicular histomorphometrics including Sertoli cell quantitation for evaluating hatchability and fertility issues in commercial breeder-broiler roosters. Poultry Science, 97(5): 1738-1747.
• Wyrobek, A.J., Eskenazi, B., Young, S., Arnheim, N., Tiemann-Boege, I., Jabs, E.W., et al. )2006(. Advancing age has differential effects on DNA damage, chromatin integrity, gene mutations, and aneuploidies in sperm. Proceedings of the National Academy of Sciences, 103(25): 9601-9606.
• Zhang, X., Berry, W.D., McDaniel, G.R., Roland, D.A., Liu, P., Calvert, C., et al. )1999(. Body weight and semen production of broiler breeder males as influenced by crude protein levels and feeding regimens during rearing. Poultry Science, 78(2): 190-196.
